VSL#3 probiotic modifies mucosal microbial composition but does not reduce colitis-associated colorectal cancer.
VSL#3 probiotic modifies mucosal microbial composition but does not reduce colitis-associated colorectal cancer.
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DOI：
10.1038/srep02868
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发表时间：
2013-10-08
影响因子：
4.6
通讯作者：
Jobin, Christian
中科院分区：
文献类型：
--
作者：
Arthur, Janelle C.;Gharaibeh, Raad Z.;Uronis, Joshua M.;Perez-Chanona, Ernesto;Sha, Wei;Tomkovich, Sarah;Muehlbauer, Marcus;Fodor, Anthony A.;Jobin, Christian
虽然益生菌已被证明在预防实验性结肠炎相关性结直肠癌(CRC)方面取得了成功，但介入治疗的有益效果相对未知。在这里，我们表明，VSL#3益生菌的介入治疗改变了肠腔和粘膜贴壁的微生物区系，但不能预防偶氮甲烷/IL10−/−小鼠模型的结肠炎相关性结肠炎的炎症或肿瘤发生。与未治疗的小鼠相比，VSL#3(109CFU/动物/天)显著增强了肿瘤的穿透性、多样性、组织学不典型增生评分和腺癌侵袭性。Illumina 16S测序表明，VSL#3显著减少了(16倍)粘膜附着微生物区系中属于梭状芽胞杆菌属的细菌分类群的丰度。通过线性模型的中介分析表明，该分类单元是VSL#3喂养的小鼠肿瘤形成增加的一个贡献因素。我们得出结论，在AOM/IL10−/−模型中，VSL#3介入治疗可以改变微生物群落组成并增强肿瘤的发生。
Although probiotics have shown success in preventing the development of experimental colitis-associated colorectal cancer (CRC), beneficial effects of interventional treatment are relatively unknown. Here we show that interventional treatment with VSL#3 probiotic alters the luminal and mucosally-adherent microbiota, but does not protect against inflammation or tumorigenesis in the azoxymethane (AOM)/Il10−/− mouse model of colitis-associated CRC. VSL#3 (109 CFU/animal/day) significantly enhanced tumor penetrance, multiplicity, histologic dysplasia scores, and adenocarcinoma invasion relative to VSL#3-untreated mice. Illumina 16S sequencing demonstrated that VSL#3 significantly decreased (16-fold) the abundance of a bacterial taxon assigned to genus Clostridium in the mucosally-adherent microbiota. Mediation analysis by linear models suggested that this taxon was a contributing factor to increased tumorigenesis in VSL#3-fed mice. We conclude that VSL#3 interventional therapy can alter microbial community composition and enhance tumorigenesis in the AOM/Il10−/− model.
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DOI：
10.1099/00221287-88-2-229
发表时间：
1975-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
JOHNSON, JL;FRANCIS, BS
通讯作者：
FRANCIS, BS
影响因子：
7.6
作者：
BARON, RM;KENNY, DA
通讯作者：
KENNY, DA
DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE
影响因子：
29.4
作者：
Gionchetti, P;Rizzello, F;Campieri, M
通讯作者：
Campieri, M