Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis
Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis
复制标题
基于变构 DNAzyme 的 DNA 逻辑电路：运算和动态分析
DOI：
10.1093/nar/gky1245
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发表时间：
2018-12
影响因子：
14.9
通讯作者：
Wei Xiaopeng
中科院分区：
文献类型：
--
作者：
Zheng Xuedong;Yang Jing;Zhou Changjun;Zhang Cheng;Zhang Qiang;Wei Xiaopeng
摘要近年来，由于DNA和酶的双重作用，DNAzyme被广泛应用于DNA电路领域，在生物工程系统、信息处理和生物计算等方面有着广泛的应用。事实上，DNA酶的活性受到亚基组装、pH控制和金属离子触发的调控。但是，这些法规要求改变整个DNA酶的序列，如分离部分和插入额外的DNA序列。受自然界蛋白质变构调节的启发，提出了一种新的变构策略，在不改变DNA序列的情况下调节DNAzyme的活性。该策略通过DNA链置换来调控DNAzyme的结构，从而达到对DNAzyme活性的控制。将该策略应用于E6型DNA酶，建立了DNA逻辑电路（YES、OR、AND、级联和反馈）的运算模型，并进行了动态仿真。变构调控具有构建更复杂分子体系的潜力，可用于生物传感和检测。
Abstract Recently, due to the dual roles of DNA and enzyme, DNAzyme has been widely used in the field of DNA circuit, which has a wide range of applications in bio-engineered system, information processing and biocomputing. In fact, the activity of DNAzymes was regulated by subunits assembly, pH control and metal ions triggers. However, those regulations required to change the sequences of whole DNAzyme, as separating parts and inserting extra DNA sequence. Inspired by the allosteric regulation of proteins in nature, a new allosteric strategy is proposed to regulate the activity of DNAzyme without DNA sequences changes. In this strategy, DNA strand displacement was used to regulate the DNAzyme structure, through which the activity of DNAzyme was well controlled. The strategy was applied to E6-type DNAzymes, and the operations of DNA logic circuit (YES, OR, AND, cascading and feedback) were established and simulated with the dynamic analyses. The allosteric regulation has potential to construct more complicated molecular systems, which can be applied to bio-sensing and detection.
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影响因子：
8.4
作者：
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