Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods.
Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods.
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无酶DNA电路对多种检测方法的有理模块化适应。
DOI：
10.1093/nar/gkr504
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发表时间：
2011-09-01
影响因子：
14.9
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Li B;Ellington AD;Chen X
信号放大是分子检测的关键组成部分。无酶信号放大对于低成本、即时诊断的发展尤其有吸引力。先前已经表明，具有信号放大能力的无酶DNA电路可以使用称为“催化发夹组装”的机制来设计。然而，目前还不清楚这种电路的效率和模块化是否适合多种分析应用。因此，我们设计并表征了基于催化发夹组装的简化DNA电路，并将其应用于多种不同的分析形式，包括荧光，比色，电化学和信号。通过优化以前基于发夹的催化组件的设计，我们发现我们的电路几乎具有零背景和高催化效率，kcat值超过1 min−1。电路的固有模块性使我们能够轻松地调整电路以检测RNA和小分子分析物。总的来说，这些数据表明催化的发夹组装适合于以“即插即用”方式进行分析物检测和信号放大。
Signal amplification is a key component of molecular detection. Enzyme-free signal amplification is especially appealing for the development of low-cost, point-of-care diagnostics. It has been previously shown that enzyme-free DNA circuits with signal-amplification capacity can be designed using a mechanism called ‘catalyzed hairpin assembly’. However, it is unclear whether the efficiency and modularity of such circuits is suitable for multiple analytical applications. We have therefore designed and characterized a simplified DNA circuit based on catalyzed hairpin assembly, and applied it to multiple different analytical formats, including fluorescent, colorimetric, and electrochemical and signaling. By optimizing the design of previous hairpin-based catalytic assemblies we found that our circuit has almost zero background and a high catalytic efficiency, with a kcat value above 1 min−1. The inherent modularity of the circuit allowed us to readily adapt our circuit to detect both RNA and small molecule analytes. Overall, these data demonstrate that catalyzed hairpin assembly is suitable for analyte detection and signal amplification in a ‘plug-and-play’ fashion.
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影响因子：
3.2
作者：
Gerasimova, Yulia V.;Cornett, Evan;Kolpashchikov, Dmitry M.
通讯作者：
Kolpashchikov, Dmitry M.
影响因子：
15
作者：
Kolpashchikov, Dmitry M.
通讯作者：
Kolpashchikov, Dmitry M.
DOI：
10.1073/pnas.0407024101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Dirks, RM;Pierce, NA
通讯作者：
Pierce, NA
影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
--
作者：
BREAKER, RR;JOYCE, GF
通讯作者：
JOYCE, GF