Isolation and Characterization of an AGAMOUS-Like Gene from Magnolia wufengensis (Magnoliaceae)
Isolation and Characterization of an AGAMOUS-Like Gene from Magnolia wufengensis (Magnoliaceae)
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五峰木兰（木兰科）无性生殖样基因的分离和鉴定
DOI：
10.1007/s11105-011-0385-3
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发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Lvyi Ma
中科院分区：
文献类型：
--
作者：
Chen Faju;Danlong Jing;Zhixiong Liu;Lvyi Ma
花同源异型 C 功能基因 AGAMOUS (AG) 通过指定雄蕊和心皮同一性、抑制 A 类基因以及调节花分生组织决定，在拟南芥发育中发挥着至关重要的作用。尽管来自其他核心真双子叶植物的 AG 同源物的功能似乎高度保守，但 AG 直向同源物在基部被子植物花结构设计中的作用仍然未知。我们从属于木兰科的木本基生被子植物五峰木兰中分离并鉴定了一个 AG 直系同源物。序列和系统发育分析表明它是euAG谱系的分支成员，因此该基因被称为MAwuAG（M. wufengensis AGAMOUS）。此外，在MAwuAG蛋白的C端区域发现了两个C蛋白特有的高度保守的基序，AG基序I和II，但在MAwuAG中没有发现通常出现在真双子叶植物euAG谱系成员中的N端延伸。 cDNA 的第一个框内 ATG 紧接在 MADS 结构域之前。半定量PCR分析表明MAwuAG的表达仅限于雄蕊和心皮的生殖器官。含有35S::MAwuAG的转基因拟南芥表现出极早的开花、更大的雄蕊和心皮以及花瓣同源转化为退化雄蕊器官，但第一轮中MAwuAG的异位表达未能将萼片转化为心皮状结构，而这种结构通常在来自其他核心真双子叶植物的AG直系同源物的过表达转基因拟南芥中观察到。此外，转基因35S::MAwuAG拟南芥的表型显示，外部三个花轮（萼片、花瓣和雄蕊）的脱落受到抑制。
The floral homeotic C function gene AGAMOUS (AG) plays crucial roles in Arabidopsis development by specifying stamen and carpel identity, repressing A-class genes, as well as regulating floral meristem determination. Although the function of AG homologs from other core eudicots appears highly conserved, the role of AG orthologs in the design of floral architecture in basal angiosperm remains unknown. We isolated and identified an AG ortholog from Magnolia wufengensis, a woody basal angiosperm belonging to the Magnoliaceae. Sequence and phylogenetic analyses revealed that it is a clade member of the euAG lineage, and hence, the gene is referred to as MAwuAG (M. wufengensis AGAMOUS). Moreover, two highly conserved motifs specific to C proteins, AG motifs I and II, are found in the C-terminal regions of the MAwuAG protein, but the N-terminal extensions that usually appear in euAG lineage members from eudicots were not found in MAwuAG. The cDNA has the first in-frame ATG immediately preceding the MADS domain. A semi-quantitative PCR analysis showed that the expression of MAwuAG was restricted to reproductive organs of stamens and carpels. The transgenic Arabidopsis containing 35S::MAwuAG displayed extremely early flowering, bigger stamens and carpels, and homeotic conversion of petals into staminoid organs, but ectopic expression of MAwuAG in the first whorls failed to convert the sepals into carpeloid structures that are usually observed in the overexpression transgenic Arabidopsis of AG orthologs from other core eudicots. In addition, the phenotype of the transgenic 35S::MAwuAG Arabidopsis revealed that the abscission of the outer three floral whorls (sepals, petals, and stamens) was inhibited.
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影响因子：
64.5
作者：
MIZUKAMI, Y;MA, H
通讯作者：
MA, H
影响因子：
--
作者：
Yamaguchi T;Hirano HY
通讯作者：
Hirano HY
DOI：
10.7525/j.issn.1673-5102.2006.01.003
发表时间：
2006-01
期刊：
Bulletin of Botanical Research
影响因子：
--
作者：
MA Lǜ-yi;Wang Luo-Rong;HE Sui-Chao;Liu Xin;Wang Xi-qun
通讯作者：
MA Lǜ-yi;Wang Luo-Rong;HE Sui-Chao;Liu Xin;Wang Xi-qun
影响因子：
--
作者：
C. Whipple;R. Schmidt
通讯作者：
C. Whipple;R. Schmidt
影响因子：
64.8
作者：
Pelaz, S;Ditta, GS;Yanofsky, MF
通讯作者：
Yanofsky, MF