Transcriptional Profile of the Industrial Hybrid Saccharomyces pastorianus Reveals Temperature-Dependent Allele Expression Bias and Preferential Orthologous Protein Assemblies.
Transcriptional Profile of the Industrial Hybrid Saccharomyces pastorianus Reveals Temperature-Dependent Allele Expression Bias and Preferential Orthologous Protein Assemblies.
复制标题
DOI：
10.1093/molbev/msab282
复制
发表时间：
2021-12-09
影响因子：
10.7
通讯作者：
Delneri D
中科院分区：
文献类型：
--
作者：
Timouma S;Balarezo-Cisneros LN;Pinto J;De La Cerda R;Bond U;Schwartz JM;Delneri D
酵母是由嗜温酿酒酵母和耐寒酿酒酵母杂交而成的一种天然酵母。这种复杂的非整倍体杂交种携带亲本等位基因的多个拷贝以及特定的杂交种基因，并编码多种蛋白质同种异构体，从而赋予新的表型，例如在低温下发酵的强大能力。这些特征导致对底物的激烈竞争和过多的生化活动，导致独特的细胞代谢。在此，我们研究了不同同源等位基因在温度变化过程中的转录特征。我们发现了温度依赖的不依赖于介质的基因，并发现35%的基因的调节依赖于细胞外亮氨酸的摄取，这表明亮氨酸代谢和温度反应之间存在相互作用。同源亲本等位基因的表达分析表明，大多数基因优先表达一个亲本等位基因而不是另一个亲本等位基因，在参与冷驯化的基因中，真巴芽孢杆菌样等位基因明显过代表。功能冗余亲本等位基因的存在可能会影响在杂交中建立的蛋白质复合物的性质，其中两个亲本等位基因都是竞争的。我们的表达数据表明，在杂交中研究的大多数蛋白质复合物可能是完全嵌合的或非特异性的，并且冗余被阻止，这种情况与基因平衡假说非常吻合。本研究首次概述了酵母的转录模式，并在表达水平上为其独特的工业性状提供了合理的解释。
Saccharomyces pastorianus is a natural yeast evolved from different hybridization events between the mesophilic S. cerevisiae and the cold-tolerant S. eubayanus. This complex aneuploid hybrid carries multiple copies of the parental alleles alongside specific hybrid genes and encodes for multiple protein isoforms which impart novel phenotypes, such as the strong ability to ferment at low temperature. These characteristics lead to agonistic competition for substrates and a plethora of biochemical activities, resulting in a unique cellular metabolism. Here, we investigated the transcriptional signature of the different orthologous alleles in S. pastorianus during temperature shifts. We identified temperature-dependent media-independent genes and showed that 35% has their regulation dependent on extracellular leucine uptake, suggesting an interplay between leucine metabolism and temperature response. The analysis of the expression of ortholog parental alleles unveiled that the majority of the genes expresses preferentially one parental allele over the other and that S. eubayanus-like alleles are significantly over-represented among the genes involved in the cold acclimatization. The presence of functionally redundant parental alleles may impact on the nature of protein complexes established in the hybrid, where both parental alleles are competing. Our expression data indicate that the majority of the protein complexes investigated in the hybrid are likely to be either exclusively chimeric or unispecific and that the redundancy is discouraged, a scenario that fits well with the gene balance hypothesis. This study offers the first overview of the transcriptional pattern of S. pastorianus and provides a rationalization for its unique industrial traits at the expression level.
登录
查看更多内容
影响因子：
9.8
作者：
Chou JY;Hung YS;Lin KH;Lee HY;Leu JY
通讯作者：
Leu JY
麦芽糖发酵糖脂酵母的微阵列核分型具有不同的麦芽三糖利用率剖面揭示了与麦芽糖和麦芽糖利用相关的基因的拷贝数变化。
影响因子：
4
作者：
Duval EH;Alves SL Jr;Dunn B;Sherlock G;Stambuk BU
通讯作者：
Stambuk BU
影响因子：
2.6
作者：
Gibson, Brian R.;Storgards, Erna;Vidgren, Virve
通讯作者：
Vidgren, Virve
DOI：
10.1111/j.1469-8137.2009.03087.x
发表时间：
2010-04
期刊：
The New phytologist
影响因子：
--
作者：
Birchler JA;Veitia RA
通讯作者：
Veitia RA
影响因子：
5.2
作者：
Ciani M;Capece A;Comitini F;Canonico L;Siesto G;Romano P
通讯作者：
Romano P