Recent advances in ion mobility-mass spectrometry for improved structural characterization of glycans and glycoconjugates.
Recent advances in ion mobility-mass spectrometry for improved structural characterization of glycans and glycoconjugates.
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DOI：
10.1016/j.cbpa.2017.10.007
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发表时间：
2018-03
影响因子：
7.8
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Chen Z;Glover MS;Li L
聚糖和糖缀合物参与调节大量的细胞和分子过程。尽管聚糖在生物学和疾病中的重要性，但由于其结构的复杂性和多样性，聚糖的表征仍然很困难。基于质谱（MS）的技术已成为表征聚糖的首要分析工具。然而，传统的基于MS的策略难以区分大量共存的异构聚糖，这些异构聚糖仅通过质量是无法区分的。正因为如此，离子迁移谱联用MS（IM-MS）作为改善聚糖表征的分析工具受到了相当大的关注，因为IM能够在MS测量之前解析异构聚糖。在这篇综述中，我们提出了最近的改进IM-MS仪器和异构体聚糖的结构表征方法。此外，我们强调了IM-MS的最新应用，说明了这项技术在各种研究领域的巨大潜力，包括糖组学，糖蛋白质组学和糖生物学。
Glycans and glycoconjugates are involved in regulating a vast array of cellular and molecular processes. Despite the importance of glycans in biology and disease, characterization of glycans remains difficult due to their structural complexity and diversity. Mass spectrometry (MS)-based techniques have emerged as the premier analytical tools for characterizing glycans. However, traditional MS-based strategies struggle to distinguish the large number of coexisting isomeric glycans that are indistinguishable by mass alone. Because of this, ion mobility spectrometry coupled to MS (IM-MS) has received considerable attention as an analytical tool for improving glycan characterization due to the capability of IM to resolve isomeric glycans prior to MS measurements. In this review, we present recent improvements in IM-MS instrumentation and methods for the structural characterization of isomeric glycans. In addition, we highlight recent applications of IM-MS that illustrate the enormous potential of this technology in a variety of research areas, including glycomics, glycoproteomics, and glycobiology.
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