Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields.
Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields.
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DOI：
10.1186/1471-2164-14-590
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发表时间：
2013-08-29
期刊：
影响因子：
4.4
通讯作者：
Lelong C
中科院分区：
文献类型：
--
作者：
Jouaux A;Lafont M;Blin JL;Houssin M;Mathieu M;Lelong C
自2008年以来，在法国观察到太平洋牡蛎（Crassostrea gigas）的幼贝和幼贝大量死亡。一种称为OsHV-1的疱疹病毒，很容易通过PCR检测到，与死亡率有关，这一点已被许多将死亡率与OsHV-1流行率联系起来的实地研究结果所证实。此外，使用病毒颗粒的实验感染已经证明了OsHV-1的致病性，但宿主对病原体的生理反应尚不清楚。本研究的目的是了解在田间感染期间对病毒发挥作用的机制。牡蛎基因组的一个主要部分，并用于研究宿主转录组死亡率在现场的微阵列分析已经开发出来。在死亡事件期间，通过微阵列比较具有和不具有可检测的OsHV-1感染的Spat分别呈现或不呈现死亡。在这项研究中，许多基因在田间对病原体感染的反应中受到调节，似乎证明了病毒在观察到的死亡率中的作用。结果允许建立一个假设的方案，宿主细胞的感染，并响应，病原体。这种反应显示了通过病毒OsHV-1生命周期的基因调节的“狭义”先天免疫，但也显示了导致宿主和病原体之间复杂相互作用的其他生物学过程。
Massive mortalities have been observed in France since 2008 on spat and juvenile Pacific oysters, Crassostrea gigas. A herpes virus called OsHV-1, easily detectable by PCR, has been implicated in the mortalities as demonstrated by the results of numerous field studies linking mortality with OsHV-1 prevalence. Moreover, experimental infections using viral particles have documented the pathogenicity of OsHV-1 but the physiological responses of host to pathogen are not well known. The aim of this study was to understand mechanisms brought into play against the virus during infection in the field. A microarray assay has been developed for a major part of the oyster genome and used for studying the host transcriptome across mortality on field. Spat with and without detectable OsHV-1 infection presenting or not mortality respectively were compared by microarray during mortality episodes. In this study, a number of genes are regulated in the response to pathogen infection on field and seems to argue to an implication of the virus in the observed mortality. The result allowed establishment of a hypothetic scheme of the host cell’s infection by, and response to, the pathogen. This response shows a “sensu stricto” innate immunity through genic regulation of the virus OsHV-1 life cycle, but also others biological processes resulting to complex interactions between host and pathogens in general.
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