Analysis of mitochondrial organization and function in the Drosophila blastoderm embryo.
Analysis of mitochondrial organization and function in the Drosophila blastoderm embryo.
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DOI：
10.1038/s41598-017-05679-1
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发表时间：
2017-07-14
影响因子：
4.6
通讯作者：
Rikhy R
中科院分区：
文献类型：
--
作者：
Chowdhary S;Tomer D;Dubal D;Sambre D;Rikhy R
线粒体在后生动物胚胎中以球状和未成熟细胞器的形式母系遗传。本研究以果蝇胚盘胚胎为材料，对其形态、分布及在胚胎发生中的作用进行了研究。我们发现线粒体相对较小、分散，并通过运动蛋白运输沿顶基轴沿着靠近微管的位置明显分布。活体成像、光漂白和光活化分析表明，它们在沿着微管的顶端-基底轴上是移动的，而在侧平面上是不移动的，从而与一个合胞体细胞相关联。光活化的线粒体在整个分裂周期中均匀地分布到子细胞中。线粒体电子传递链（ETC）的药理学和遗传学抑制导致ATP耗竭，激活AMPK并减少合胞中期沟延伸。总之，我们发现，小而分散的线粒体的果蝇胚盘胚胎定位微管运输和提供ATP的快速合胞体分裂周期。我们的研究打开了使用果蝇胚胎发生作为模型系统的可能性，以研究母体线粒体形态和代谢突变对胚胎图案化和分化的影响。
Mitochondria are inherited maternally as globular and immature organelles in metazoan embryos. We have used the Drosophila blastoderm embryo to characterize their morphology, distribution and functions in embryogenesis. We find that mitochondria are relatively small, dispersed and distinctly distributed along the apico-basal axis in proximity to microtubules by motor protein transport. Live imaging, photobleaching and photoactivation analyses of mitochondrially targeted GFP show that they are mobile in the apico-basal axis along microtubules and are immobile in the lateral plane thereby associating with one syncytial cell. Photoactivated mitochondria distribute equally to daughter cells across the division cycles. ATP depletion by pharmacological and genetic inhibition of the mitochondrial electron transport chain (ETC) activates AMPK and decreases syncytial metaphase furrow extension. In summary, we show that small and dispersed mitochondria of the Drosophila blastoderm embryo localize by microtubule transport and provide ATP locally for the fast syncytial division cycles. Our study opens the possibility of use of Drosophila embryogenesis as a model system to study the impact of maternal mutations in mitochondrial morphology and metabolism on embryo patterning and differentiation.
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影响因子：
9.2
作者：
De Vos, KJ;Allan, VJ;Sheetz, MP
通讯作者：
Sheetz, MP
DOI：
10.1083/jcb.200601156
发表时间：
2006-04-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Frescas D;Mavrakis M;Lorenz H;Delotto R;Lippincott-Schwartz J
通讯作者：
Lippincott-Schwartz J
DOI：
10.1083/jcb.200601067
发表时间：
2006-05-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Glater EE;Megeath LJ;Stowers RS;Schwarz TL
通讯作者：
Schwarz TL
影响因子：
12.4
作者：
Davila, D.;Connolly, N. M. C.;Prehn, J. H. M.
通讯作者：
Prehn, J. H. M.
影响因子：
2.7
作者：
Coffman, JA;McCarthy, JJ;Robertson, AJ
通讯作者：
Robertson, AJ