Myeloid dendritic cells display downregulation of C-type lectin receptors and aberrant lectin uptake in systemic lupus erythematosus.
Myeloid dendritic cells display downregulation of C-type lectin receptors and aberrant lectin uptake in systemic lupus erythematosus.
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DOI：
10.1186/ar2517
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发表时间：
2008
影响因子：
4.9
通讯作者：
Kaplan MJ
中科院分区：
文献类型：
--
作者：
Monrad SU;Rea K;Thacker S;Kaplan MJ
There is a growing body of evidence implicating aberrant dendritic cell function as a crucial component in the immunopathogenesis of systemic lupus erythematosus. The purpose of the present study was to characterize the phagocytic capacity and expression of receptors involved in pathogen recognition and self-nonself discrimination on myeloid dendritic cells from patients with lupus. Unstimulated or stimulated monocyte-derived dendritic cells were obtained from lupus patients and healthy control individuals, and expression of C-type lectin receptors (mannose receptor and dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin), complement-receptor 3 and Fcγ receptors was determined by flow cytometry. Dextran uptake by lupus and control dendritic cells was also assessed by flow cytometry. Serum IFNγ was quantified by ELISA, and uptake of microbial products was measured using fluorescently labeled zymosan. When compared with dendritic cells from healthy control individuals, unstimulated and stimulated lupus dendritic cells displayed significantly decreased dextran uptake and mannose receptor and dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin expression. Decreased expression of the mannose receptor was associated with high serum IFNγ levels, but not with maturation status or medications. Diminished dextran uptake and mannose receptor expression correlated with lupus disease activity. There were no differences between control and lupus dendritic cells in the expression of other pattern recognition receptors or in the capacity to uptake zymosan particles Lupus dendritic cells have diminished endocytic capacity, which correlates with decreased mannose receptor expression. While this phenomenon appears primarily intrinsic to dendritic cells, modulation by serum factors such as IFNγ could play a role. These abnormalities may be relevant to the aberrant immune homeostasis and the increased susceptibility to infections described in lupus.
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影响因子：
4.4
作者：
Bajtay, Z;Csomor, E;Erdei, A
通讯作者：
Erdei, A
影响因子：
64.5
作者：
Geijtenbeek, TBH;Torensma, R;Figdor, CG
通讯作者：
Figdor, CG
DOI：
10.1084/jem.172.6.1785
发表时间：
1990-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ezekowitz RA;Sastry K;Bailly P;Warner A
通讯作者：
Warner A
影响因子：
5.5
作者：
Decker, Patrice;Koetter, Ina;Rammensee, Hans-Georg
通讯作者：
Rammensee, Hans-Georg
影响因子：
15.9
作者：
MARODI, L;SCHREIBER, S;JOHNSTON, RB
通讯作者：
JOHNSTON, RB