The Separation and Quantitation of Peptides with and without Oxidation of Methionine and Deamidation of Asparagine Using Hydrophilic Interaction Liquid Chromatography with Mass Spectrometry (HILIC-MS).
The Separation and Quantitation of Peptides with and without Oxidation of Methionine and Deamidation of Asparagine Using Hydrophilic Interaction Liquid Chromatography with Mass Spectrometry (HILIC-MS).
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DOI：
10.1007/s13361-016-1565-z
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发表时间：
2017-05
影响因子：
3.2
通讯作者：
Orlando R
中科院分区：
文献类型：
--
作者：
Badgett MJ;Boyes B;Orlando R
Peptides with deamidated asparagine residues and oxidized methionine residues are often not resolved sufficiently to allow quantitation of their native and modified forms using reversed phase (RP) chromatography. The accurate quantitation of these modifications is vital in protein biotherapeutic analysis because they can affect a protein’s function, activity, and stability. We demonstrate here that hydrophilic interaction liquid chromatography (HILIC) adequately and predictably separates peptides with these modifications from their native counterparts. Furthermore, coefficients describing the extent of the hydrophilicity of these modifications have been derived and were incorporated into a previously made peptide retention prediction model that is capable of predicting the retention times of peptides with and without these modifications.
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DOI：
10.1016/s0021-9673(00)96972-3
发表时间：
1990-01-19
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ALPERT, AJ
通讯作者：
ALPERT, AJ
DOI：
10.1073/pnas.231472998
发表时间：
2001-11-06
影响因子：
11.1
作者：
Moskovitz, J;Bar-Noy, S;Stadtman, ER
通讯作者：
Stadtman, ER
DOI：
10.1073/pnas.032671199
发表时间：
2002-03-05
影响因子：
11.1
作者：
Ruan, H;Tang, XD;Hoshi, T
通讯作者：
Hoshi, T
影响因子：
5.3
作者：
Khawli, Leslie A.;Goswami, Sirj;Motchnik, Paul
通讯作者：
Motchnik, Paul
影响因子：
4.7
作者：
Gabbita, SP;Aksenov, MY;Markesbery, WR
通讯作者：
Markesbery, WR