A micropatterning and image processing approach to simplify measurement of cellular traction forces.
A micropatterning and image processing approach to simplify measurement of cellular traction forces.
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DOI：
10.1016/j.actbio.2011.08.013
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发表时间：
2012-01
影响因子：
9.7
通讯作者：
Smith, Michael L.
中科院分区：
文献类型：
--
作者：
Polio, Samuel R.;Rothenberg, Katheryn E.;Stamenovic, Dimitrije;Smith, Michael L.
量化细胞对其周围环境施加的牵引力对于我们理解癌症，发育和基础细胞生物学的进步至关重要。这一领域是通过开发工程细胞培养系统，允许光学测量细胞介导的位移和计算算法，允许这些位移转化为应力和力成为可能。在这里，我们提出了一个新的进步牵引力显微镜聚丙烯酰胺（PAA）凝胶，解决现有技术的局限性。通过间接图案化技术，我们生成了在规则化阵列中具有荧光1 μm点标记的PAA凝胶。这改进了现有的牵引力测量，因为（i）可以在一个实验中测量多个视场，而不需要去除单元;（ii）牵引力矢量被建模为离散点力，而不是连续场，使用我们已经在线提供的非常简单的计算算法;和（iii）图案转移技术适用于任何公开的用于在玻璃上产生图案的技术。在未来，这种技术将被用于测量复杂的模式与多个，空间上不同的配体在系统中的牵引力施加应变的基板，并在三明治培养，产生准三维环境的细胞。
Quantification of the traction forces that cells apply to their surroundings has been critical to the advancement of our understanding of cancer, development and basic cell biology. This field was made possible through the development of engineered cell culture systems that permit optical measurement of cell-mediated displacements and computational algorithms that allow conversion of these displacements into stresses and forces. Here, we present a novel advancement of traction force microscopy on polyacrylamide (PAA) gels that addresses limitations of existing technologies. Through an indirect patterning technique, we generated PAA gels with fluorescent 1 μm dot markers in a regularized array. This improves existing traction measurements since (i) multiple fields of view can be measured in one experiment without the need for cell removal; (ii) traction vectors are modeled as discrete point forces, and not as a continuous field, using an extremely simple computational algorithm that we have made available online; and (iii) the pattern transfer technique is amenable to any of the published techniques for producing patterns on glass. In the future, this technique will be used for measuring traction forces on complex patterns with multiple, spatially distinct ligands in systems for applying strain to the substrate, and in sandwich cultures that generate quasi-three-dimensional environments for cells.
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影响因子：
7.8
作者：
Gardel, Margaret L.;Sabass, Benedikt;Ji, Lin;Danuser, Gaudenz;Schwarz, Ulrich S.;Waterman, Clare M.
通讯作者：
Waterman, Clare M.
影响因子：
3.4
作者：
Sugita, Shukei;Adachi, Taiji;Sato, Masaaki
通讯作者：
Sato, Masaaki
DOI：
10.1073/pnas.0408482102
发表时间：
2005-02-15
影响因子：
11.1
作者：
du Roure, O;Saez, A;Ladoux, B
通讯作者：
Ladoux, B
影响因子：
3.4
作者：
Sabass, Benedikt;Gardel, Margaret L.;Schwarz, Ulrich S.
通讯作者：
Schwarz, Ulrich S.
DOI：
10.1073/pnas.0914547107
发表时间：
2010-06-01
影响因子：
11.1
作者：
Liu, Zhijun;Tan, John L.;Chen, Christopher S.
通讯作者：
Chen, Christopher S.