Recent Emergence of Bovine Coronavirus Variants with Mutations in the Hemagglutinin-Esterase Receptor Binding Domain in U.S. Cattle.
Recent Emergence of Bovine Coronavirus Variants with Mutations in the Hemagglutinin-Esterase Receptor Binding Domain in U.S. Cattle.
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DOI：
10.3390/v14102125
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发表时间：
2022-09-27
期刊：
影响因子：
--
通讯作者：
Hause BM
中科院分区：
文献类型：
--
作者：
Workman AM;McDaneld TG;Harhay GP;Das S;Loy JD;Hause BM
牛冠状病毒（BCoV）已经蔓延到许多物种，包括人类，其中宿主范围变异冠状病毒OC 43是地方性的。表面刺突（S）和血凝素酯酶（HE）糖蛋白的相对活性的平衡控制BCoV的亲合力，这对种间传播和宿主适应至关重要。在这里，78个基因组直接从2013年至2022年从12个州（主要是美国中西部）收集的牛的临床样本中进行测序，观察到相对较少的遗传多样性，基因组具有>98%的核苷酸同一性。2020年至2022年期间从四个州（内布拉斯加州、科罗拉多、加州和威斯康星州）收集的11株分离株在HE基因的受体结合域（RBD）中含有12个核苷酸的插入，与中国最近报道的类似，2020年从内布拉斯加州收集的一个单一基因组在HE基因RBD中含有一个新的12个核苷酸缺失。在HE RBD中含有插入或缺失的同基因HE蛋白保持酯酶活性，并且可以结合牛颌下粘蛋白，一种富含受体9-O-乙酰化-唾液酸的底物，尽管建模预测了对受体结合至关重要的HE R3环的结构变化。在RBD中出现具有结构变体的BCoV提高了进一步种间传播的可能性。
Bovine coronavirus (BCoV) has spilled over to many species, including humans, where the host range variant coronavirus OC43 is endemic. The balance of the opposing activities of the surface spike (S) and hemagglutinin-esterase (HE) glycoproteins controls BCoV avidity, which is critical for interspecies transmission and host adaptation. Here, 78 genomes were sequenced directly from clinical samples collected between 2013 and 2022 from cattle in 12 states, primarily in the Midwestern U.S. Relatively little genetic diversity was observed, with genomes having >98% nucleotide identity. Eleven isolates collected between 2020 and 2022 from four states (Nebraska, Colorado, California, and Wisconsin) contained a 12 nucleotide insertion in the receptor-binding domain (RBD) of the HE gene similar to one recently reported in China, and a single genome from Nebraska collected in 2020 contained a novel 12 nucleotide deletion in the HE gene RBD. Isogenic HE proteins containing either the insertion or deletion in the HE RBD maintained esterase activity and could bind bovine submaxillary mucin, a substrate enriched in the receptor 9-O-acetylated-sialic acid, despite modeling that predicted structural changes in the HE R3 loop critical for receptor binding. The emergence of BCoV with structural variants in the RBD raises the possibility of further interspecies transmission.
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DOI：
10.1016/j.cvfa.2008.10.013
发表时间：
2009-03
期刊：
The Veterinary clinics of North America. Food animal practice
影响因子：
--
作者：
Foster DM;Smith GW
通讯作者：
Smith GW
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
10.7
作者：
Lam HM;Ratmann O;Boni MF
通讯作者：
Boni MF
影响因子：
3.7
作者：
Hause BM;Collin EA;Anderson J;Hesse RA;Anderson G
通讯作者：
Anderson G
影响因子：
5.4
作者：
Alekseev, Konstantin P.;Vlasova, Anastasia N.;Saif, Linda J.
通讯作者：
Saif, Linda J.