Bovine rhinitis viruses are common in U.S. cattle with bovine respiratory disease.
Bovine rhinitis viruses are common in U.S. cattle with bovine respiratory disease.
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DOI：
10.1371/journal.pone.0121998
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Anderson G
中科院分区：
文献类型：
--
作者：
Hause BM;Collin EA;Anderson J;Hesse RA;Anderson G
牛鼻炎病毒（BRV）是牛呼吸道综合征的病原体，但几十年来对其流行病学和生态学的研究很少。在美国，仅鉴定了牛鼻炎A病毒1（BRAV 1），而牛鼻炎A病毒2（BRAV 2）和牛鼻炎B病毒（BRBV）先前仅分别在英国和日本鉴定。对来自堪萨斯的牛呼吸道疾病（BRD）诊断提交材料的鼻拭子进行宏基因组测序，鉴定出与BRAV 2和BRBV具有约90%核苷酸相似性的重叠群。使用参考基因组的从头组装和模板组装的组合产生了接近完整的BRAV 2和BRBV基因组。牛鼻炎A病毒1（BRAV 1）的近完整的基因组也确定了历史分离，使进一步的分子流行病学研究。设计了一种靶向3D聚合酶基因的5 '-核酸酶逆转录PCR方法，并用于筛选204份存档的BRD临床标本。13例（6.4%）为阳性。6份阳性样本的宏基因组测序鉴定出5份样本的混合BRAV 1/BRAV 2、BRAV 1/BRBV和BRAV 2/BRBV感染。一份样本显示仅感染BRAV 1。使用细胞培养适应BRBV的血清阳性率研究发现，免疫荧光试验反应性抗体在分析的牛群中很常见。总之，这些结果表明，BRV感染在患有呼吸道疾病的牛中很常见，BRAV 1、BRAV 2和BRBV在美国牛中共同传播，与30多年前从不同地点分离的病毒具有高度相似性。
Bovine rhinitis viruses (BRV) are established etiological agents of bovine respiratory disease complex however little research into their epidemiology and ecology has been published for several decades. In the U.S., only bovine rhinitis A virus 1 (BRAV1) has been identified while bovine rhinitis A virus 2 (BRAV2) and bovine rhinitis B virus (BRBV) were previously only identified in England and Japan, respectively. Metagenomic sequencing of a nasal swab from a bovine respiratory disease (BRD) diagnostic submission from Kansas identified contigs with approximately 90% nucleotide similarity to BRAV2 and BRBV. A combination of de novo and templated assemblies using reference genomes yielded near complete BRAV2 and BRBV genomes. The near complete genome of bovine rhinitis A virus 1 (BRAV1) was also determined from a historical isolate to enable further molecular epidemiological studies. A 5’-nuclease reverse transcription PCR assay targeting the 3D polymerase gene was designed and used to screen 204 archived BRD clinical specimens. Thirteen (6.4%) were positive. Metagenomic sequencing of six positive samples identified mixed BRAV1/BRAV2, BRAV1/BRBV and BRAV2/BRBV infections for five samples. One sample showed infection only with BRAV1. Seroprevalence studies using a cell culture adapted BRBV found immunofluorescence assay-reactive antibodies were common in the herds analyzed. Altogether, these results demonstrate that BRV infections are common in cattle with respiratory disease and that BRAV1, BRAV2 and BRBV co-circulate in U.S. cattle and have high similarity to viruses isolated more than 30 years ago from diverse locations.
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影响因子：
3.7
作者：
Hollister, Jason R.;Vagnozzi, Ariel;Rieder, Elizabeth
通讯作者：
Rieder, Elizabeth
DOI：
10.1073/pnas.0504666102
发表时间：
2005-09-06
影响因子：
11.1
作者：
Allander, T;Tammi, MT;Andersson, B
通讯作者：
Andersson, B
DOI：
10.1007/bf01249864
发表时间：
1972-01-01
期刊：
ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG
影响因子：
--
作者：
IDE, PR;DARBYSHIRE, JH
通讯作者：
DARBYSHIRE, JH
DOI：
10.3181/00379727-128-33140
发表时间：
1968-01-01
影响因子：
--
作者：
MOHANTY, SB;LILLIE, MG
通讯作者：
LILLIE, MG
DOI：
10.1007/bf01249865
发表时间：
1972-01-01
期刊：
ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG
影响因子：
--
作者：
IDE, PR;DARBYSHIRE, JH
通讯作者：
DARBYSHIRE, JH