A Ruthenium(II) Complex as a Luminescent Probe for DNA Mismatches and Abasic Sites.
A Ruthenium(II) Complex as a Luminescent Probe for DNA Mismatches and Abasic Sites.
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DOI：
10.1021/acs.inorgchem.7b01037
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发表时间：
2017-07-17
影响因子：
4.6
通讯作者：
Barton JK
中科院分区：
文献类型：
--
作者：
Boynton AN;Marcélis L;McConnell AJ;Barton JK
[Ru(Bpy)2(BNIQ)]2+(BNIQ=苯并[c][1，7]萘啶-1-异喹啉)是一种高选择性的DNA错配和碱基结合探针，与配对良好的碱基对相比，对这些不稳定区域具有500倍的结合亲和力。由于这种更高的结合亲和力，与完全匹配的DNA相比，在含有单个碱基错配或基本位点的DNA双链存在时，该络合物表现出增强的稳态发射。铜(Phen)22+和[Fe(CN)6]3-的发光猝灭实验表明，通过金属插入，络合物从小槽到失配结合。该配合物对不同单碱基错配的发射响应优先结合到更不稳定的错配，也与金属插入结合一致。这项工作表明，通过合理设计具有空间膨胀的芳香族配体的络合物，可以实现对双链DNA中不稳定区域的高选择性。发光络合物[Ru(Bpy)2(BNIQ)]2+选择性地针对双链DNA中的失配和基本位置，并且在这些缺陷位置的存在下表现出相对于完全匹配的碱基对的增强的发射强度。
[Ru(bpy)2(BNIQ)]2+ (BNIQ = Benzo[c][1,7]naphthyridine-1-isoquinoline), which incorporates the sterically expansive BNIQ ligand, is a highly selective luminescent probe for DNA mismatches and abasic sites, possessing a 500-fold higher binding affinity toward these destabilized regions relative to well-matched base pairs. As a result of this higher binding affinity, the complex exhibits an enhanced steady-state emission in the presence of DNA duplexes containing a single base mismatch or abasic site compared to fully well-matched DNA. Luminescence quenching experiments with Cu(phen)22+ and [Fe(CN)6]3– implicate binding of the complex to a mismatch from the minor groove via metalloinsertion. The emission response of the complex to different single base mismatches, binding preferentially to the more destabilized mismatches, is also consistent with binding by metalloinsertion. This work shows that high selectivity toward destabilized regions in duplex DNA can be achieved through the rational design of a complex with a sterically expansive aromatic ligand. The luminescent complex [Ru(bpy)2(BNIQ)]2+ selectivity targets mismatched and abasic sites in duplex DNA and exhibits an enhanced emission intensity in the presence of these defect sites relative to well-matched base pairs.
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影响因子：
2.8
作者：
Boyle KM;Barton JK
通讯作者：
Barton JK
影响因子：
15
作者：
BARTON, JK;DANISHEFSKY, AT;GOLDBERG, JM
通讯作者：
GOLDBERG, JM
影响因子：
2.9
作者：
Jackson, BA;Barton, JK
通讯作者：
Barton, JK
影响因子：
15
作者：
Milne, JE;Buchwald, SL
通讯作者：
Buchwald, SL
影响因子：
20.6
作者：
Baggaley, Elizabeth;Weinstein, Julia A.;Williams, J. A. Gareth
通讯作者：
Williams, J. A. Gareth