A simple automated approach to measure mouse whole gut transit.
A simple automated approach to measure mouse whole gut transit.
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DOI：
10.1111/nmo.13994
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发表时间：
2021-03
影响因子：
3.5
通讯作者：
Beyder A
中科院分区：
文献类型：
--
作者：
Kacmaz H;Alto A;Knutson K;Linden DR;Gibbons SJ;Farrugia G;Beyder A
胃肠道运动是一个复杂的生理过程，对胃肠道的正常功能至关重要。胃肠道动力的破坏经常发生在胃肠道疾病和治疗的副作用。小鼠全肠道传输测量，如胭脂红前沿传输，形成体内临床前GI运动研究的基石。我们已经开发出一种易于实现，节省劳动力的方法来测量小鼠的全肠道通过时间。这种方法使用廉价的市售材料，通过高清摄像机捕捉延时视频进行离线分析，来监控颗粒生产。我们描述了我们的自动肠道传输设置的组装，并通过比较传统的传输测量与洛哌丁胺的传输延迟来验证这种方法。我们证明，与对照组相比，洛哌丁胺组转运减慢，表现为总颗粒产生减少和全肠道转运时间延长。与文献报道的结果相比，对照组的通过时间延长。通过每隔10-15分钟破坏笼以模拟常规方法，全肠道通过率加速至与文献相当的时间，表明破坏影响测定并支持使用自动化方法。一种新型的自动化、廉价且易于组装的全肠道运输装置，与传统方法相比，该装置节省劳动力，并且对动物行为的干扰最小。
Gastrointestinal (GI) motility is a complex physiological process critical for normal GI function. Disruption of GI motility occurs frequently in GI diseases and as side-effects of therapeutics. Whole gut transit measurements in mice, like carmine red leading-edge transit, form the cornerstone of in vivo preclinical GI motility studies. We have developed an easily achievable, labor-saving method to measure whole gut transit time in mice. This approach uses inexpensive, commercially available materials to monitor pellet production over time via high definition cameras capturing timelapse video for offline analysis. We describe the assembly of our automated gut transit setup and validate this approach by comparing conventional transit measurements with transit delay by loperamide. We demonstrate that compared to the control group, the loperamide group had slowed transit, evidenced by a decrease in total pellet production and prolonged whole gut transit time. The control group had an extended transit time compared with the results reported in the literature. Whole gut transit rates accelerated to times comparable to the literature by disrupting cages every 10–15 min to imitate the conventional approach, suggesting that disruption affects the assay and supports the use of an automated approach. A novel automated, inexpensive, and easily assembled whole gut transit setup is labor-saving and allows minimal disruption to animal behavior compared to the conventional approach.
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DOI：
10.1523/jneurosci.6684-10.2011
发表时间：
2011-06-15
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Li Z;Chalazonitis A;Huang YY;Mann JJ;Margolis KG;Yang QM;Kim DO;Côté F;Mallet J;Gershon MD
通讯作者：
Gershon MD
影响因子：
3.2
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通讯作者：
Golay X
DOI：
10.1016/j.giec.2008.12.005
发表时间：
2009-01-01
影响因子：
--
作者：
Parkman, Henry P;Orr, William C
通讯作者：
Orr, William C
DOI：
10.1152/ajpgi.00317.2007
发表时间：
2007-11-01
影响因子：
4.5
作者：
Choi, Kyoung Moo;Zhu, Jin;Farrugia, Gianrico
通讯作者：
Farrugia, Gianrico
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影响因子：
4.2
作者：
Odamaki T;Kato K;Sugahara H;Hashikura N;Takahashi S;Xiao JZ;Abe F;Osawa R
通讯作者：
Osawa R