Resistant starches protect against colonic DNA damage and alter microbiota and gene expression in rats fed a Western diet.
Resistant starches protect against colonic DNA damage and alter microbiota and gene expression in rats fed a Western diet.
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DOI：
10.3945/jn.111.147660
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Topping DL
中科院分区：
文献类型：
--
作者：
Conlon MA;Kerr CA;McSweeney CS;Dunne RA;Shaw JM;Kang S;Bird AR;Morell MK;Lockett TJ;Molloy PL;Regina A;Toden S;Clarke JM;Topping DL
以高直链玉米淀粉（HAMS）或丁酰化玉米淀粉（HAMSB）形式饲喂抗性淀粉（RS），对抗膳食蛋白诱导的大鼠结肠细胞DNA损伤。在这项研究中，大鼠喂食含有可消化淀粉[低直链淀粉玉米淀粉（LAMS）或低直链淀粉全麦（LAW）]或RS [HAMS、HAMSB或通过RNA干扰产生的全高直链淀粉小麦（HAW）]的中等脂肪（19%）和蛋白质（20%）的西式饮食11周（n = 10/组）。对照饮食包括7%脂肪、13%蛋白质和LAMS。结肠细胞DNA单链断裂（SSB）显着更高（70%），在大鼠喂食西方饮食含有LAMS相对于对照组。饮食HAW，HAMS和HAMSB反对这种影响，同时提高SCFA的Nesta水平，降低氨和苯酚水平。SSB与总大肠SCFA呈负相关，包括结肠丁酸浓度（R2 = 0.40; P = 0.009），与结肠氨浓度呈正相关（R2 = 0.40; P = 0.014）。使用系统发育微阵列对肠道微生物群进行分析，揭示了分为3个不同组的概况：对照组和LAMS; HAMS和HAMSB;以及LAW和HAW。与维持基因组完整性相关的结肠基因（特别是Mdm 2，Top1，Msh 3，Ung，Rere，Cebpa，Gmnn和Parg）的表达随RS来源而改变和变化。HAW在对抗大鼠饮食诱导的结肠DNA损伤方面与HAMS和HAMSB一样有效，但它们对大肠微生物群和结肠细胞基因表达的影响不同，可能是由于HAW中存在其他纤维组分。
Resistant starch (RS), fed as high amylose maize starch (HAMS) or butyrylated HAMS (HAMSB), opposes dietary protein-induced colonocyte DNA damage in rats. In this study, rats were fed Western-type diets moderate in fat (19%) and protein (20%) containing digestible starches [low amylose maize starch (LAMS) or low amylose whole wheat (LAW)] or RS [HAMS, HAMSB, or a whole high amylose wheat (HAW) generated by RNA interference] for 11 wk (n = 10/group). A control diet included 7% fat, 13% protein, and LAMS. Colonocyte DNA single-strand breaks (SSB) were significantly higher (by 70%) in rats fed the Western diet containing LAMS relative to controls. Dietary HAW, HAMS, and HAMSB opposed this effect while raising digesta levels of SCFA and lowering ammonia and phenol levels. SSB correlated inversely with total large bowel SCFA, including colonic butyrate concentration (R2 = 0.40; P = 0.009), and positively with colonic ammonia concentration (R2 = 0.40; P = 0.014). Analysis of gut microbiota populations using a phylogenetic microarray revealed profiles that fell into 3 distinct groups: control and LAMS; HAMS and HAMSB; and LAW and HAW. The expression of colonic genes associated with the maintenance of genomic integrity (notably Mdm2, Top1, Msh3, Ung, Rere, Cebpa, Gmnn, and Parg) was altered and varied with RS source. HAW is as effective as HAMS and HAMSB in opposing diet-induced colonic DNA damage in rats, but their effects on the large bowel microbiota and colonocyte gene expression differ, possibly due to the presence of other fiber components in HAW.
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影响因子：
1.6
作者：
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通讯作者：
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