Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis.
Signal transduction and amplification through enzyme-triggered ligand release and accelerated catalysis.
复制标题
DOI：
10.1039/c5sc01588j
复制
发表时间：
2015-08-01
期刊：
影响因子：
8.4
通讯作者：
Frost CG
中科院分区：
文献类型：
--
作者：
Goggins S;Marsh BJ;Lubben AT;Frost CG
通过设计一种新型酶底物描述了酶触发的催化信号放大级联，该酶底物一旦被触发，就会选择性地激活有机金属转移氢化催化剂。信号转导和信号放大都是生物信号传导途径中使用的重要机制。受这个过程的启发，我们开发了一种信号放大方法，利用酶的选择性和高活性以及有机金属催化剂的稳健性和通用性，实现生物和合成催化剂级联的混合。配体酶底物被设计为在酶存在的情况下选择性自焚，释放配体，该配体可以与金属预催化剂结合并加速转移氢化反应的速率。然后将酶触发的催化信号放大应用于一系列催化剂底物，证明信号放大和信号转导都可以通过这种方法实现。
An enzyme-triggered catalytic signal amplification cascade is described through the design of a novel enzyme substrate that selectively activates an organometallic transfer hydrogenation catalyst once triggered. Signal transduction and signal amplification are both important mechanisms used within biological signalling pathways. Inspired by this process, we have developed a signal amplification methodology that utilises the selectivity and high activity of enzymes in combination with the robustness and generality of an organometallic catalyst, achieving a hybrid biological and synthetic catalyst cascade. A proligand enzyme substrate was designed to selectively self-immolate in the presence of the enzyme to release a ligand that can bind to a metal pre-catalyst and accelerate the rate of a transfer hydrogenation reaction. Enzyme-triggered catalytic signal amplification was then applied to a range of catalyst substrates demonstrating that signal amplification and signal transduction can both be achieved through this methodology.
登录
查看更多内容
增强的比色免疫测定结合酶级联放大策略，用于低丰度蛋白质的超灵敏检测。
影响因子：
4.6
作者：
Gao Z;Hou L;Xu M;Tang D
通讯作者：
Tang D
影响因子：
16.6
作者：
de Groot, FMH;Albrecht, C;Scheeren, HW
通讯作者：
Scheeren, HW
影响因子：
15
作者：
Liu, JW;Lu, Y
通讯作者：
Lu, Y
影响因子：
21.8
作者：
Hull, Jonathan F.;Himeda, Yuichiro;Fujita, Etsuko
通讯作者：
Fujita, Etsuko
影响因子：
3.3
作者：
Karton-Lifshin, Naama;Shabat, Doron
通讯作者：
Shabat, Doron