Basis for enhanced barrier function of pigmented skin.
Basis for enhanced barrier function of pigmented skin.
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DOI：
10.1038/jid.2014.187
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发表时间：
2014-09
影响因子：
6.5
通讯作者：
Elias, Peter M.
中科院分区：
文献类型：
--
作者：
Man, Mao-Qiang;Lin, Tzu-Kai;Santiago, Juan L.;Celli, Anna;Zhong, Lily;Huang, Zhi-Ming;Roelandt, Truus;Hupe, Melanie;Sundberg, John P.;Silva, Kathleen A.;Crumrine, Debra;Martin-Ezquerra, Gemma;Trullas, Caries;Sun, Richard;Wakefield, Joan S.;Wei, Maria L.;Feingold, Kenneth R.;Mauro, Theodora M.;Elias, Peter M.
与浅色皮肤的人相比，深色皮肤的人显示出上级渗透屏障功能。深色皮肤的角质层（SC）的pH值降低可以解释增强的功能，因为酸化浅色人类SC将屏障功能重置为深色水平。在SKH 1（非色素）与SKH 2/J（色素）无毛小鼠中，我们评估了色素依赖性pH值降低如何影响表皮屏障功能。与SKH 1小鼠相比，SKH 2/J小鼠的渗透屏障稳态增强，这与与黑色素颗粒挤出共定位的下SC中pH降低相关。深色的人表皮也显示出外表皮中大量的黑色素挤出。急性屏障破坏和局部碱性pH挑战都加速了SKH 2/J（但不是SKH 1）SC的再酸化，同时诱导黑色素挤出。SKH 2/J小鼠还显示SC酸化酶分泌型磷脂酶A2 f（sPLA 2f）的表达增强。SKH 2/J小鼠屏障功能的增强可能归因于两种酸性pH依赖性神经酰胺生成酶β-葡萄糖脑苷脂酶和酸性鞘磷脂酶的活性增强，导致SC板层双层的加速成熟。最后，与浅色培养物相比，深色着色的人角质形成细胞的器官型培养物显示增强的屏障功能。总之，这些结果表明，着色表皮的上级屏障功能可以在很大程度上归因于黑色素持久性/挤出的pH降低影响和增强的sPLA 2f表达。
Humans with darkly-pigmented skin display superior permeability barrier function in comparison to humans with lightly-pigmented skin. The reduced pH of the stratum corneum (SC) of darkly-pigmented skin could account for enhanced function, because acidifying lightly-pigmented human SC resets barrier function to darkly-pigmented levels. In SKH1 (non-pigmented) vs. SKH2/J (pigmented) hairless mice, we evaluated how a pigment-dependent reduction in pH could influence epidermal barrier function. Permeability barrier homeostasis is enhanced in SKH2/J vs. SKH1 mice, correlating with a reduced pH in the lower SC that co-localizes with the extrusion of melanin granules. Darkly-pigmented human epidermis also shows substantial melanin extrusion in the outer epidermis. Both acute barrier disruption and topical basic pH challenges accelerate re-acidification of SKH2/J (but not SKH1) SC, while inducing melanin extrusion. SKH2/J mice also display enhanced expression of the SC acidifying enzyme, secretory phospholipase A2f (sPLA2f). Enhanced barrier function of SKH2/J mice could be attributed to enhanced activity of two acidic pH-dependent, ceramide-generating enzymes, β-glucocerebrosidase and acidic sphingomyelinase, leading to accelerated maturation of SC lamellar bilayers. Finally, organotypic cultures of darkly-pigmented-bearing human keratinocytes display enhanced barrier function in comparison to lightly-pigmented cultures. Together, these results suggest that the superior barrier function of pigmented epidermis can be largely attributed to the pH-lowering impact of melanin persistence/extrusion and enhanced sPLA2f expression.
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影响因子：
6.5
作者：
Hachem, Jean-Pierre;Roelandt, Truus;Elias, Peter M.
通讯作者：
Elias, Peter M.
DOI：
10.1111/j.1600-0781.1997.tb00214.x
发表时间：
1997-08-01
影响因子：
2.6
作者：
Holleran, WM;Uchida, Y;Elias, PM
通讯作者：
Elias, PM
影响因子：
6.5
作者：
Hong, Seung P.;Kim, Min J.;Choi, Eung H.
通讯作者：
Choi, Eung H.
DOI：
10.1038/jid.2008.442
发表时间：
2009-07
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.9
作者：
Elias, Peter M.;Menon, Gopinathan;Wetzel, Bruce K.;Williams, John (Jack) W.
通讯作者：
Williams, John (Jack) W.