Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability.
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability.
复制标题
DOI：
10.1371/journal.pntd.0005677
复制
发表时间：
2017-07
影响因子：
3.8
通讯作者：
Dimopoulos G
中科院分区：
文献类型：
--
作者：
Short SM;Mongodin EF;MacLeod HJ;Talyuli OAC;Dimopoulos G
蚊子中肠微生物区系已被证明影响媒介对多种人类病原体的能力。微生物区系在现场是高度可变的，这种变异性的来源还不清楚，这限制了我们理解或预测其对病原体传播的影响的能力。在这项工作中，我们报告了对登革热病毒易感性不同的埃及伊蚊菌株之间雌性成年中肠细菌载量的显著差异。这些菌株之间的中肠微生物组组成总体上是相似的，81-92%的Read来自相同的五个细菌科，尽管我们确实检测到一些细菌科的存在差异，包括黄杆菌科和内细菌科。我们对细菌载量差异最大的两个蚊子品系进行了转录转录分析，发现它们在许多与氨基酸代谢有关的基因，特别是支链氨基酸降解途径的转录丰度上存在差异。然后，我们通过使用RNA干扰针对多个基因来沉默这一途径，这导致了菌株特异性的细菌增殖，从而消除了菌株之间中肠细菌负荷的差异。这表明支链氨基酸(BCAA)降解途径控制着中肠细菌负荷，尽管其机制尚不清楚。总体而言，我们的结果表明，氨基酸代谢可以影响中肠微生物区系。此外，他们认为支链氨基酸降解途径活性的遗传或生理变化可能在一定程度上解释了田间中肠微生物区系的变化。蚊虫中肠微生物区系在蚊虫对人类病原体的易感性中起着重要作用，是蚊病传播的重要组成部分。然而，微生物区系可能是高度可变的，这种变异的来源还没有很好地了解。在这项工作中，我们的目标是提高我们对蚊子影响其微生物区系的方式的理解。为了做到这一点，我们利用了中肠微生物负荷不同的埃及伊蚊菌株。我们比较了两个细菌负荷差异很大的菌株的转录本，发现参与氨基酸代谢(特别是支链氨基酸降解)的基因在菌株之间受到不同的调控。我们发现，当我们使用RNA干扰方法沉默这些基因时，细菌载量以菌株特有的方式增加，证实了这些基因在控制中肠微生物区系的增殖中的作用。总体而言，这些结果表明，蚊子体内氨基酸代谢或分解代谢的差异可能对蚊子微生物群有重要影响。
The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmission. In this work, we report significant variation in female adult midgut bacterial load between strains of A. aegypti which vary in their susceptibility to dengue virus. Composition of the midgut microbiome was similar overall between the strains, with 81–92% of reads coming from the same five bacterial families, though we did detect differences in the presence of some bacterial families including Flavobacteriaceae and Entobacteriaceae. We conducted transcriptomic analysis on the two mosquito strains that showed the greatest difference in bacterial load, and found that they differ in transcript abundance of many genes implicated in amino acid metabolism, in particular the branched chain amino acid degradation pathway. We then silenced this pathway by targeting multiple genes using RNA interference, which resulted in strain-specific bacterial proliferation, thereby eliminating the difference in midgut bacterial load between the strains. This suggests that the branched chain amino acid (BCAA) degradation pathway controls midgut bacterial load, though the mechanism underlying this remains unclear. Overall, our results indicate that amino acid metabolism can act to influence the midgut microbiota. Moreover, they suggest that genetic or physiological variation in BCAA degradation pathway activity may in part explain midgut microbiota variation in the field. The mosquito midgut microbiota plays an important role in mosquito susceptibility to human pathogens and therefore is an important component of mosquito disease transmission. The microbiota can be highly variable, however, and the sources of this variation are not well understood. In this work, we aimed to improve our understanding of the ways in which the mosquito can influence its microbiota. To do this, we utilized strains of Aedes aegypti that vary in their midgut microbial load. We compared the transcriptomes of two strains with highly disparate bacterial loads, and found that genes involved in amino acid metabolism (specifically branched chain amino acid degradation) were differentially regulated between the strains. We found that when we silenced these genes using an RNA interference approach, bacterial loads increased in a strain-specific manner, confirming a role for these genes in controlling proliferation of the midgut microbiota. Overall, these results suggest that differences in amino acid metabolism or catabolism in the mosquito could have important implications for the mosquito microbiota.
登录
查看更多内容
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Apte-Deshpande A;Paingankar M;Gokhale MD;Deobagkar DN
通讯作者：
Deobagkar DN
影响因子：
4.2
作者：
Alvarez-Perez, Sergio;Herrera, Carlos M.;de Vega, Clara
通讯作者：
de Vega, Clara
影响因子：
4.1
作者：
Esslinger SM;Schwalb B;Helfer S;Michalik KM;Witte H;Maier KC;Martin D;Michalke B;Tresch A;Cramer P;Förstemann K
通讯作者：
Förstemann K
影响因子：
2
作者：
Charan, Shakti S.;Pawar, Kiran D.;Shouche, Yogesh S.
通讯作者：
Shouche, Yogesh S.