Serratia odorifera a midgut inhabitant of Aedes aegypti mosquito enhances its susceptibility to dengue-2 virus.
Serratia odorifera a midgut inhabitant of Aedes aegypti mosquito enhances its susceptibility to dengue-2 virus.
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DOI：
10.1371/journal.pone.0040401
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Deobagkar DN
中科院分区：
文献类型：
--
作者：
Apte-Deshpande A;Paingankar M;Gokhale MD;Deobagkar DN
蚊子中肠在媒介易感性和病原体相互作用中起着至关重要的作用。因此，鉴定中肠环境中可持续的微生物群落有助于评估其对蚊子-病原体相互作用的贡献，进而有助于评估媒介能力。为了了解埃及伊蚊中肠细菌多样性，我们对野外采集和实验室培养的埃及伊蚊进行了肠道微生物筛选研究。本研究表明，伊蚊幼虫和成虫的微生物区系。埃及伊蚊中肠结构复杂，以革兰氏阴性变形菌为主。在野外采集和实验室饲养的幼虫和成虫中发现了稳定的气味沙雷菌。通过将该菌与DENV-2共投喂成伊蚊，评估该可持续肠道微生物对DENV-2易感性的潜在影响。埃及伊蚊雌性（无肠道菌群）。观察结果显示，与单独饲喂登革热-2和另一种肠道居民氧化微杆菌相比，雌伊蚊对病毒的易感性显著增强。基于本研究结果，我们提出了白纹伊蚊对DENV-2敏感性的增强。雌性埃及伊蚊的死亡是由于雌性埃及伊蚊肠道中表面的禁止素分子被寄生在肠道中的臭蚊多肽阻断。
Mosquito midgut plays a crucial role in its vector susceptibility and pathogen interaction. Identification of the sustainable microflora of the midgut environment can therefore help in evaluating its contribution in mosquito-pathogen interaction and in turn vector competence. To understand the bacterial diversity in the midgut of Aedes aegypti mosquitoes, we conducted a screening study of the gut microbes of these mosquitoes which were either collected from fields or reared in the laboratory “culture-dependent” approach. This work demonstrated that the microbial flora of larvae and adult Ae. aegypti midgut is complex and is dominated by Gram negative proteobacteria. Serratia odorifera was found to be stably associated in the midguts of field collected and laboratory reared larvae and adult females. The potential influence of this sustainable gut microbe on DENV-2 susceptibility of this vector was evaluated by co-feeding S. odorifera with DENV-2 to adult Ae. aegypti females (free of gut flora). The observations revealed that the viral susceptibility of these Aedes females enhanced significantly as compared to solely dengue-2 fed and another gut inhabitant, Microbacterium oxydans co-fed females. Based on the results of this study we proposed that the enhancement in the DENV-2 susceptibility of Ae. aegypti females was due to blocking of prohibitin molecule present on the midgut surface of these females by the polypeptide of gut inhabitant S. odorifera.
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DOI：
10.1111/j.1582-4934.2006.tb00404.x
发表时间：
2006-04
影响因子：
5.3
作者：
Mishra S;Murphy LC;Murphy LJ
通讯作者：
Murphy LJ
DOI：
10.1080/00034983.1974.11686929
发表时间：
1974-01-01
影响因子：
--
作者：
MCGREEVY, PB;MCCLELLAND, GA;LAVOIPIERRE, MM
通讯作者：
LAVOIPIERRE, MM
影响因子：
3.7
作者：
Kuadkitkan, Atichat;Wikan, Nitwara;Smith, Duncan R.
通讯作者：
Smith, Duncan R.
DOI：
10.4269/ajtmh.1996.54.219
发表时间：
1996-02-01
影响因子：
3.3
作者：
DeMaio, J;Pumpuni, CB;Beier, JC
通讯作者：
Beier, JC
DOI：
10.1073/pnas.0610451104
发表时间：
2007-05-22
影响因子：
11.1
作者：
Favia, Guido;Ricci, Irene;Daffonchio, Daniele
通讯作者：
Daffonchio, Daniele