Controlling scaffold conductivity and pore size to direct myogenic cell alignment and differentiation.
Controlling scaffold conductivity and pore size to direct myogenic cell alignment and differentiation.
复制标题
DOI：
10.1002/jbm.a.37418
复制
发表时间：
2022-10
影响因子：
4.9
通讯作者：
Caliari, Steven R.
中科院分区：
文献类型：
--
作者：
Basurto, Ivan M.;Muhammad, Samir A.;Gardner, Gregg M.;Christ, George J.;Caliari, Steven R.
骨骼肌的三维（3D）各向异性和电兴奋性的组合对于实现正常运动至关重要。我们以前开发了一种3D对齐的胶原蛋白支架，结合导电聚吡咯（PPy）颗粒来概括这些关键的肌肉特性，并表明与非导电对照相比，该支架有助于增强肌管成熟。为了进一步优化这种支架设计，这项工作评估了导电聚合物掺入和支架孔结构对肌原性细胞行为的影响。合成导电PPy和聚（3，4-亚乙基二氧噻吩）（PEDOT）颗粒，并在定向冷冻干燥之前将其混合到I型胶原和硫酸软骨素的悬浮液中，以制备各向异性支架。能量色散光谱显示导电PEDOT颗粒在整个支架中均匀分布，导致电导率增加三倍，同时支持与非导电支架相似的成肌细胞代谢活性。冷冻温度的控制使得能够制造具有98至238 μm的孔径范围的PEDOT掺杂的支架。成肌细胞符合各向异性的接触指导线索独立的孔径显示纵向细胞骨架排列。较小孔支架的增加的比表面积有助于挽救在较大孔导电支架中观察到的成肌细胞代谢活性的初始降低，同时还促进成肌标记物肌球蛋白重链（MHC）的表达水平适度增加和成肌细胞决定蛋白（MyoD）的基因表达。然而，与较大孔隙的变体相比，支架中心的细胞浸润略有减少。这些数据共同强调了对齐和PEDOT掺杂的胶原支架促进肌原性细胞组织化和分化的潜力。
Skeletal muscle's combination of three‐dimensional (3D) anisotropy and electrical excitability is critical for enabling normal movement. We previously developed a 3D aligned collagen scaffold incorporating conductive polypyrrole (PPy) particles to recapitulate these key muscle properties and showed that the scaffold facilitated enhanced myotube maturation compared with nonconductive controls. To further optimize this scaffold design, this work assessed the influence of conductive polymer incorporation and scaffold pore architecture on myogenic cell behavior. Conductive PPy and poly(3,4‐ethylenedioxythiophene) (PEDOT) particles were synthesized and mixed into a suspension of type I collagen and chondroitin sulfate prior to directional freeze‐drying to produce anisotropic scaffolds. Energy dispersive spectroscopy revealed homogenous distribution of conductive PEDOT particles throughout the scaffolds that resulted in a threefold increase in electrical conductivity while supporting similar myoblast metabolic activity compared to nonconductive scaffolds. Control of freezing temperature enabled fabrication of PEDOT‐doped scaffolds with a range of pore diameters from 98 to 238 μm. Myoblasts conformed to the anisotropic contact guidance cues independent of pore size to display longitudinal cytoskeletal alignment. The increased specific surface area of the smaller pore scaffolds helped rescue the initial decrease in myoblast metabolic activity observed in larger pore conductive scaffolds while also promoting modestly increased expression levels of the myogenic marker myosin heavy chain (MHC) and gene expression of myoblast determination protein (MyoD). However, cell infiltration to the center of the scaffolds was marginally reduced compared with larger pore variants. Together these data underscore the potential of aligned and PEDOT‐doped collagen scaffolds for promoting myogenic cell organization and differentiation.
登录
查看更多内容
DOI：
10.1098/rsta.2005.1678
发表时间：
2006-01-15
影响因子：
5
作者：
Ashby, MF
通讯作者：
Ashby, MF
影响因子：
9.7
作者：
Ferlin, Kimberly M.;Prendergast, Margaret E.;Fisher, John P.
通讯作者：
Fisher, John P.
DOI：
10.1016/j.jmbbm.2018.09.012
发表时间：
2019-03
影响因子：
3.9
作者：
Divakar P;Yin K;Wegst UGK
通讯作者：
Wegst UGK
影响因子：
3.4
作者：
Gillies, Allison R.;Lieber, Richard L.
通讯作者：
Lieber, Richard L.
影响因子：
7.1
作者：
Nishimura, Takanori
通讯作者：
Nishimura, Takanori