An Integrative Genomic Island Affects the Adaptations of the Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii to High Temperature and High Hydrostatic Pressure.
An Integrative Genomic Island Affects the Adaptations of the Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii to High Temperature and High Hydrostatic Pressure.
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整合基因组岛影响嗜压超耐热古菌火球菌 yayanosii 对高温和高静水压的适应
DOI：
10.3389/fmicb.2016.01927
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发表时间：
2016
影响因子：
5.2
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Li Z;Li X;Xiao X;Xu J
深海热液喷口环境的特点是高静水压力和急剧的温度和化学梯度。水平基因转移被认为在微生物适应这种极端环境中起着重要作用。在这项研究中，一个21.4 kb的DNA片段被确定为一个基因组岛，指定PYG 1，在嗜压超嗜热菌Pyrococcus yayanosii的基因组序列。根据序列比对和功能注释，PYG 1基因可初步划分为5个模块，其功能分别与迁移、DNA修复、代谢过程和毒素-抗毒素系统有关。整合酶可介导PYG 1基因在弥野早疫病菌A1染色体上的定点整合和切除。用SimR盒对PYG 1的基因置换是成功的。在不同pH、盐度、温度和静水压力等胁迫条件下，比较了突变株Δ PYG 1及其亲本菌株P. yayanosii A2的生长情况。Δ PYG 1突变株在100°C下生长时表现出降低的生长，而Δ PYG 1的生物量在80 MPa下培养时显著增加。在不同的温度和压力条件下，观察到PYG 1的模块III中的基因的差异表达。这项研究证明了古细菌整合基因组岛的第一个例子，可能会影响超嗜热嗜压菌P. yayanosii对高温和高静水压力的适应。
Deep-sea hydrothermal vent environments are characterized by high hydrostatic pressure and sharp temperature and chemical gradients. Horizontal gene transfer is thought to play an important role in the microbial adaptation to such an extreme environment. In this study, a 21.4-kb DNA fragment was identified as a genomic island, designated PYG1, in the genomic sequence of the piezophilic hyperthermophile Pyrococcus yayanosii. According to the sequence alignment and functional annotation, the genes in PYG1 could tentatively be divided into five modules, with functions related to mobility, DNA repair, metabolic processes and the toxin-antitoxin system. Integrase can mediate the site-specific integration and excision of PYG1 in the chromosome of P. yayanosii A1. Gene replacement of PYG1 with a SimR cassette was successful. The growth of the mutant strain ΔPYG1 was compared with its parent strain P. yayanosii A2 under various stress conditions, including different pH, salinity, temperature, and hydrostatic pressure. The ΔPYG1 mutant strain showed reduced growth when grown at 100°C, while the biomass of ΔPYG1 increased significantly when cultured at 80 MPa. Differential expression of the genes in module III of PYG1 was observed under different temperature and pressure conditions. This study demonstrates the first example of an archaeal integrative genomic island that could affect the adaptation of the hyperthermophilic piezophile P. yayanosii to high temperature and high hydrostatic pressure.
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影响因子：
4.4
作者：
Michels, PC;Clark, DS
通讯作者：
Clark, DS
广泛的噬菌体流产感染系统通过IV型毒素 - 抗毒素机制发挥作用。
影响因子：
14.9
作者：
Dy RL;Przybilski R;Semeijn K;Salmond GP;Fineran PC
通讯作者：
Fineran PC
DOI：
10.1099/ijs.0.024653-0
发表时间：
2011-12-01
影响因子：
2.8
作者：
Birrien, Jean-Louis;Zeng, Xiang;Prieur, Daniel
通讯作者：
Prieur, Daniel
嗜压超嗜热古菌 Pyrococcus yayanosii 的遗传工具
影响因子：
2.9
作者：
Li, Xuegong;Fu, Ling;Xu, Jun
通讯作者：
Xu, Jun
影响因子：
11.3
作者：
Juhas M;van der Meer JR;Gaillard M;Harding RM;Hood DW;Crook DW
通讯作者：
Crook DW