Transcriptional profile of Taxus chinensis cells in response to methyl jasmonate.
Transcriptional profile of Taxus chinensis cells in response to methyl jasmonate.
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红豆杉细胞响应茉莉酸甲酯的转录谱
DOI：
10.1186/1471-2164-13-295
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发表时间：
2012-07-02
期刊：
影响因子：
4.4
通讯作者：
Yu LJ
中科院分区：
文献类型：
--
作者：
Li ST;Zhang P;Zhang M;Fu CH;Zhao CF;Dong YS;Guo AY;Yu LJ
背景
茉莉酸甲酯 (MeJA) 已成功用作有效的引发剂，以提高培养的红豆杉细胞中紫杉醇和其他紫杉烷的产量。然而，MeJA 介导的紫杉烷生物合成的机制仍不清楚。目前无法获得红豆杉属物种的基因组信息。因此，为了更好地探索 MeJA 介导的紫杉烷生物合成的生物学机制，需要有关红豆杉细胞转录组的信息，特别是对 MeJA 响应的基因表达变化的描述。
结果
在这项研究中，通过“RNA-seq”分析了MeJA处理后16小时（T16）的红豆杉细胞和模拟处理的细胞（T0）的转录组谱，以研究红豆杉细胞响应MeJA诱导的转录变化。两个样本的 cDNA 生成了超过 5800 万个读数（长度为 200 bp），并发现了 46,581 个单基因。发现两个时间点之间存在差异表达的基因有 13,469 个，包括所有已知的茉莉酸 (JA) 生物合成/JA 信号通路基因和紫杉醇相关基因。 qRT-PCR结果显示，随机选取的12个DEG和10个紫杉醇生物合成基因的表达谱与RNA-Seq数据一致。 MeJA 似乎可以刺激涉及多个相关功能类别的大量基因，例如植物激素生物合成和苯丙素生物合成。此外，许多编码转录因子的基因被证明对 MeJA 诱导有反应。
结论
转录组分析结果表明，外源应用MeJA可以诱导JA生物合成/JA信号通路/防御反应，激活一系列转录因子，并增加负责紫杉醇合成的萜类生物合成通路中基因的表达。这种对基因表达信息的全面描述可以极大地促进我们对红豆杉细胞中 MeJA 介导的紫杉烷生物合成的分子机制的理解。
Background
Methyl jasmonate (MeJA) has been successfully used as an effective elicitor to enhance production of taxol and other taxanes in cultured Taxus cells. However the mechanism of MeJA-mediated taxane biosynthesis remains unclear. Genomic information for species in the genus Taxus is currently unavailable. Therefore, information about the transcriptome of Taxus cells and specifically, description of changes in gene expression in response to MeJA, is needed for the better exploration of the biological mechanisms of MeJA-mediated taxane biosynthesis.
Results
In this research, the transcriptome profiles of T. chinensis cells at 16 hours (T16) after MeJA treatment and of mock-treated cells (T0) were analyzed by “RNA-seq” to investigate the transcriptional alterations of Taxus cell in response to MeJA elicitation. More than 58 million reads (200 bp in length) of cDNA from both samples were generated, and 46,581 unigenes were found. There were 13,469 genes found to be expressed differentially between the two timepoints, including all of the known jasmonate (JA) biosynthesis/JA signaling pathway genes and taxol-related genes. The qRT-PCR results showed that the expression profiles of 12 randomly selected DEGs and 10 taxol biosynthesis genes were found to be consistent with the RNA-Seq data. MeJA appeared to stimulate a large number of genes involved in several relevant functional categories, such as plant hormone biosynthesis and phenylpropanoid biosynthesis. Additionally, many genes encoding transcription factors were shown to respond to MeJA elicitation.
Conclusions
The results of a transcriptome analysis suggest that exogenous application of MeJA could induce JA biosynthesis/JA signaling pathway/defence responses, activate a series of transcription factors, as well as increase expression of genes in the terpenoid biosynthesis pathway responsible for taxol synthesis. This comprehensive description of gene expression information could greatly facilitate our understanding of the molecular mechanisms of MeJA-mediated taxane biosynthesis in Taxus cells.
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影响因子：
7
作者：
Li, Ruiqiang;Zhu, Hongmei;Wang, Jun
通讯作者：
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影响因子：
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作者：
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作者：
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