Post-injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury.
Post-injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury.
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DOI：
10.1002/nau.23120
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发表时间：
2017-06
影响因子：
2
通讯作者：
Yoshimura N
中科院分区：
文献类型：
--
作者：
Wada N;Shimizu T;Takai S;Shimizu N;Kanai AJ;Tyagi P;Kakizaki H;Yoshimura N
目的：探讨每日不同次数的膀胱挤压对脊髓损伤小鼠膀胱功能障碍的影响。雌性C57BL/6N小鼠于第8/9天脊髓横断。连续4周，每天手工挤压膀胱排出尿液。根据挤压膀胱的次数将小鼠分为三组；A：每天一次，B：每天两次，C：每天三次。横断后4周，在清醒状态下进行单次充液膀胱术。测定膀胱黏膜NGF、L6/S1背根神经节（DRG） P2X受体和TRP通道mRNA表达量。C组膀胱重量小于a组。膀胱容量、空后残余量、在存储阶段和非void收缩明显大与B组相比,A组C或C组的神经生长因子水平都低于A组或B P2X3的表达TRPA1在B组和C组相比降低A P2X2是减少的表达具有抑制受损膀胱管理延伸组B组相比,A SCI后增加数量的日常改善储存和排泄的膀胱排空膀胱功能障碍与膀胱NGF减少以及腰骶DRG中P2X和TRP转录物减少有关。
To examine the effects of a different number of daily bladder squeezes on bladder dysfunction in mice with spinal cord injury (SCI). Spinal cord was transected at the Th8/9 in female C57BL/6N mice. Their bladders were manually squeezed to eliminate urine inside every day for 4 weeks. The mice were divided into three groups depending on the number of bladder squeezes; A: once daily, B: twice daily, C: three times daily. Four weeks after transection, single-filling cystometry were performed under an awake condition. NGF in the bladder mucosa and mRNA expression of P2X receptors and TRP channels in L6/S1 dorsal root ganglia (DRG) were measured. Bladder weight in group C was less than that of group A. Bladder capacity, post-void residual, and the number of non-voiding contractions during the storage phase were significantly larger in group A compared to group B or C. The level of NGF in groups C were lower compared to group A or B. The expression of P2X3 and TRPA1 in groups B and C was decreased compared to group A. The expression of P2X2 was decreased in groups B compared to group A. The post-injury bladder management after SCI with an increased number of daily bladder emptying improves the storage and voiding bladder dysfunction associated with the reduction of NGF in the bladder as well as P2X and TRP transcripts in lumbosacral DRG.
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影响因子：
6.6
作者：
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通讯作者：
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