In situ seed baiting to isolate germination-enhancing fungi for an epiphytic orchid, Dendrobium aphyllum (Orchidaceae)
In situ seed baiting to isolate germination-enhancing fungi for an epiphytic orchid, Dendrobium aphyllum (Orchidaceae)
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原位种子诱饵分离附生兰花石斛兰（兰科）的发芽促进真菌
DOI：
10.1007/s00572-014-0565-8
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发表时间：
2014-02
期刊：
影响因子：
3.9
通讯作者：
Jiang-Yun Gao
中科院分区：
文献类型：
--
作者：
Chun-Ling Sheng;Uromi Manage Goodale;Shi-Cheng Shao;Jiang-Yun Gao
兰花保护工作，使用种子和物种特异性真菌，支持种子发芽，需要分离，鉴定和共生真菌的发芽促进测试。然而，很少有研究集中在发展这种技术的附生植物，构成大多数兰花。在这项研究中，进行了西双版纳热带植物园，云南，中国，我们使用的种子石斛，当地濒危和药用价值的附生兰花，吸引发芽促进真菌。从种子诱饵中分离到的两种真菌中，Tulasnella spp.和木霉属，Tulasnella对种子萌发、原球茎形成和幼苗发育的促进作用分别为13.6%、85.7%和45.2%（P < 0.0001）.从文心兰种子中分离到的另一种促进种子萌发的真菌-根盘菌（Epulorhiza）也能促进种子萌发（6.5%; P < 0.05）和原球茎形成（20.3%; P < 0.0001），而木霉菌则抑制种子萌发26.4%（P < 0.0001）。Tulasnella是唯一产生幼苗的处理。光照促进了种子吸胀、原球茎形成和二叶种子发育（P < 0.0001）。由于种子萌发阶段的成功不依赖于真菌，我们建议引入Tulasnella以促进D。在原球茎形成阶段促进无叶植物种子萌发，并且提供光以促进萌发以及幼苗进一步发育。我们的研究结果表明，原位种子诱饵可以用来分离种子萌发促进真菌的发展，为保护和重新引入的努力附生兰花，如D。无翅
Orchid conservation efforts, using seeds and species-specific fungi that support seed germination, require the isolation, identification, and germination enhancement testing of symbiotic fungi. However, few studies have focused on developing such techniques for the epiphytes that constitute the majority of orchids. In this study, conducted in Xishuangbanna Tropical Botanical Garden, Yunnan, China, we used seeds of Dendrobium aphyllum, a locally endangered and medicinally valuable epiphytic orchid, to attract germination promoting fungi. Of the two fungi isolated from seed baiting, Tulasnella spp. and Trichoderma spp., Tulasnella, enhanced seed germination by 13.6 %, protocorm formation by 85.7 %, and seedling development by 45.2 % (all P < 0.0001). Epulorhiza, another seed germination promoting fungi isolated from Cymbidium mannii, also enhanced seed germination (6.5 %; P < 0.05) and protocorm formation (20.3 %; P < 0.0001), but Trichoderma suppressed seed germination by 26.4 % (P < 0.0001). Tulasnella was the only treatment that produced seedlings. Light increased seed imbibition, protocorm formation, and two-leaved seed development of Tulasnella inoculated seeds (P < 0.0001). Because the germination stage success was not dependent on fungi, we recommend that Tulasnella be introduced for facilitating D. aphyllum seed germination at the protocorm formation stage and that light be provided for increasing germination as well as further seedling development. Our findings suggest that in situ seed baiting can be used to isolate seed germination-enhancing fungi for the development of seedling production for conservation and reintroduction efforts of epiphytic orchids such as D. aphyllum.
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影响因子：
3.9
作者：
H. Shimura;Mai Sadamoto;Mayumi Matsuura;T. Kawahara;S. Naito;Y. Koda
通讯作者：
H. Shimura;Mai Sadamoto;Mayumi Matsuura;T. Kawahara;S. Naito;Y. Koda
影响因子：
9.4
作者：
MASUHARA, G;KATSUYA, K
通讯作者：
KATSUYA, K
影响因子：
1.8
作者：
Stewart, SL;Zettler, LW
通讯作者：
Zettler, LW
DOI：
--
发表时间：
1996
期刊：
--
影响因子：
--
作者：
E. Hágsater;V. Dumont;A. Pridgeon
通讯作者：
E. Hágsater;V. Dumont;A. Pridgeon
影响因子：
17.3
作者：
H. Seliger
通讯作者：
H. Seliger