Degradation of Veterinary Antibiotics in Swine Manure via Anaerobic Digestion.
Degradation of Veterinary Antibiotics in Swine Manure via Anaerobic Digestion.
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DOI：
10.3390/bioengineering7040123
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发表时间：
2020-10-09
期刊：
影响因子：
--
通讯作者：
Vo PH
中科院分区：
文献类型：
--
作者：
Hosseini Taleghani A;Lim TT;Lin CH;Ericsson AC;Vo PH
抗生素耐药微生物因其对人类健康的严重和不可逆转的后果而引起人们的极大关注。畜牧业被认为负有责任，部分原因是每年使用的抗生素数量巨大。本研究对兽用抗生素(VA)的降解、去除阈值的确定以及金霉素(CTC)和泰乐菌素联合作用对消化过程的影响进行了研究。利用实验室规模的厌氧消化器研究了猪粪中VA的潜在缓解作用。消化器的工作体积为1.38 L(装在1.89-L玻璃瓶中)，水力停留时间为21天，负荷率为1.0g-vs L−1d−1。消化器在孵化器中保持在39±2℃，每隔2天加载一次，每4天产生一次沼气，每周测定消化器的pH值。在添加VAS之前，允许厌氧消化(AD)过程稳定在1.5~2HRT。试验比较了VAS对粪便养分、挥发性固体去除、VA降解和沼气产量的影响。添加到粪便样品中的VA浓度分别为2 6 3~2 98 mg/L和88~2 6 3 mg/L。对AD处理前后的VA浓度进行分析，以确定VA的降解率。还进行了额外的试验，以确认在室温和消化器温度下溶解在水中的VAS的降解。由于VA的加入，沼气产量和操作变量出现了一定的波动。即使在水溶液中储存6天后，所有的四氯化碳都被发现降解；因此，没有基线来估计AD的影响。至于泰乐菌素，由于AD的作用，其降解率为100%(与12天的水溶液储存中观察到的24-40%的降解率相比，降解率为100%)。此外，在用两种VAS混合处理的豆渣样品中也观察到泰乐菌素的完全降解。最后，利用50个变异最大的操作分类单元(OTU)S对每一组进行了扩增序列测定，并检测到了组间的完美区分。研究了VAS给药时间和剂量对黄曲霉变形杆菌、协生菌和类杆菌的影响。这些生物标志物的丰度可以用来预测样本的治疗组。
Antibiotic-resistant microorganisms are drawing a lot of attention due to their severe and irreversible consequences on human health. The animal industry is considered responsible in part because of the enormous volume of antibiotics used annually. In the current research, veterinary antibiotic (VA) degradation, finding the threshold of removal and recognizing the joint effects of chlortetracycline (CTC) and Tylosin combination on the digestion process were studied. Laboratory scale anaerobic digesters were utilized to investigate potential mitigation of VA in swine manure. The digesters had a working volume of 1.38 L (in 1.89-L glass jar), with a hydraulic retention time (HRT) of 21 days and a loading rate of 1.0 g-VS L−1 d−1. Digesters were kept at 39 ± 2 °C in incubators and loaded every two days, produced biogas every 4 days and digester pH were measured weekly. The anaerobic digestion (AD) process was allowed 1.5 to 2 HRT to stabilize before adding the VAs. Tests were conducted to compare the effects of VAs onto manure nutrients, volatile solid removal, VA degradation, and biogas production. Concentrations of VA added to the manure samples were 263 to 298 mg/L of CTC, and 88 to 263 mg/L of Tylosin, respectively. Analysis of VA concentrations before and after the AD process was conducted to determine the VA degradation. Additional tests were also conducted to confirm the degradation of both VAs dissolved in water under room temperature and digester temperature. Some fluctuations of biogas production and operating variables were observed because of the VA addition. All CTC was found degraded even only after 6 days of storage in water solution; thus, there was no baseline to estimate the effects of AD. As for Tylosin, 100% degradation was observed due to the AD (removal was 100%, compared with 24–40% degradation observed in the 12-day water solution storage). Besides, complete Tylosin degradation was also observed in the digestate samples treated with a mixture of the two VAs. Lastly, amplicon sequencing was performed on each group by using the 50 most variable operational taxonomic units (OTUs)s and perfect discriminations were detected between groups. The effect of administration period and dosage of VAs on Phyla Firmicutes Proteobacteria, Synergistetes and Phylum Bacteroides was investigated. These biomarkers’ abundance can be employed to predict the sample’s treatment group.
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影响因子：
11.4
作者：
Garcia-Pena, E. I.;Parameswaran, P.;Krajmalnik-Brown, R.
通讯作者：
Krajmalnik-Brown, R.
影响因子：
2.7
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影响因子：
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通讯作者：
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影响因子：
2.7
作者：
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通讯作者：
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