Expression of a maize NBS gene ZmNBS42 enhances disease resistance in Arabidopsis
Expression of a maize NBS gene ZmNBS42 enhances disease resistance in Arabidopsis
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玉米NBS基因ZmNBS42的表达增强拟南芥的抗病性
DOI：
10.1007/s00299-018-2324-3
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发表时间：
2018-07
影响因子：
6.2
通讯作者：
Li Xiaoyu
中科院分区：
文献类型：
--
作者：
Xu Yunjian;Liu Fang;Zhu Suwen;Li Xiaoyu
ZmNBS42 在拟南芥植物中的表达赋予了对细菌病原体的抗性，为进一步遗传育种中的玉米抗性增强提供了潜在的可能性。核苷酸结合位点 (NBS) 结构域蛋白在抗病性中发挥着关键作用。在本研究中，我们从玉米中分离出一个新的NBS基因ZmNBS42，并系统地研究了其抗病功能。我们发现，玉米叶片中 ZmNBS42 的表达水平因接种玉米赤霉和 SA 处理而显着增加。空间表达模式分析表明，在发育过程中，ZmNBS42在玉米根、叶、茎、节间和种子中普遍高表达，而在果皮和胚中低表达。为了更好地理解 ZmNBS42 的作用，我们在异源系统中过表达 ZmNBS42。本塞姆氏烟草叶子中 ZmNBS42 的瞬时过度表达会诱导过敏反应。 ZmNBS42 过表达（ZmNBS42-OE）拟南芥植物比 Col-0 植物产生更多的 SA 含量，并且与 Col-0 植物相比，一些防御响应基因的表达水平增加。此外，ZmNBS42-OE拟南芥植物对丁香假单胞菌番茄致病变种DC3000 (Pst DC3000)表现出增强的抗性。这些结果表明ZmNBS42可以作为抗病性的重要调节因子，因此更好地了解ZmNBS42将有利于玉米育种计划中抗病性的增强。
Expression of the ZmNBS42 in Arabidopsis plants conferred resistance to bacterial pathogens, providing potential resistance enhancement of maize in further genetic breeding. Nucleotide-binding site (NBS) domain proteins play critical roles in disease resistance. In this study, we isolate a novel NBS gene ZmNBS42 from maize and systematically investigate its function on disease resistance. We find that the expression levels of ZmNBS42 in maize leaf were strikingly increased in response to Bipolaris maydis inoculation and SA treatment. The spatial expression pattern analysis reveals that, during development, ZmNBS42 is ubiquitously highly expressed in maize root, leaf, stem, internode and seed, but lowly expressed in pericarp and embryo. To better understand the roles of ZmNBS42, we overexpressed ZmNBS42 in heterologous systems. Transient overexpression of ZmNBS42 in the leaves of Nicotiana benthamiana induces a hypersensitive response. ZmNBS42 overexpression (ZmNBS42-OE) Arabidopsis plants produced more SA content than Col-0 plants, and increased the expression levels of some defense-responsive genes compared to Col-0 plants. Moreover, the ZmNBS42-OE Arabidopsis plants displayed enhanced resistance against Pseudomonas syringae pathovar tomato DC3000 (Pst DC3000). These results together suggest that ZmNBS42 can serve as an important regulator in disease resistance, thus better understanding of ZmNBS42 would benefit the resistance enhancement in maize breeding programs.
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影响因子：
27.5
作者：
Liu, Wei;Frick, Michele;Laroche, Andre
通讯作者：
Laroche, Andre
DOI：
10.1007/978-1-62703-194-3_13
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Cho, Kyoungwon;Han, Oksoo;Rakwal, Randeep
通讯作者：
Rakwal, Randeep
影响因子：
2.8
作者：
Gao, Yulong;Xu, Zhaoli;Lu, Xiuping
通讯作者：
Lu, Xiuping
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.0503023102
发表时间：
2005-10
影响因子：
11.1
作者：
Bingyu Zhao;Xing-hua Lin;J. Poland;H. Trick;J. Leach;S. Hulbert
通讯作者：
Bingyu Zhao;Xing-hua Lin;J. Poland;H. Trick;J. Leach;S. Hulbert