A New High Throughput Screening Platform for Cell Encapsulation in Alginate Hydrogel Shows Improved Hepatocyte Functions by Mesenchymal Stromal Cells Co-encapsulation.
A New High Throughput Screening Platform for Cell Encapsulation in Alginate Hydrogel Shows Improved Hepatocyte Functions by Mesenchymal Stromal Cells Co-encapsulation.
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DOI：
10.3389/fmed.2018.00216
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发表时间：
2018
影响因子：
3.9
通讯作者：
Filippi C
中科院分区：
文献类型：
--
作者：
Iansante V;Dhawan A;Masmoudi F;Lee CA;Fernandez-Dacosta R;Walker S;Fitzpatrick E;Mitry RR;Filippi C
肝细胞移植已成为肝脏疾病肝移植的替代方案。已经提出将包封在藻酸盐微珠中的肝细胞用于治疗急性肝功能衰竭，因为它们能够在肝脏再生的同时提供肝功能。此外，它们不需要免疫抑制，因为藻酸盐保护肝细胞免受受体免疫细胞的侵害。间充质基质细胞是再生医学中非常有吸引力的候选者，能够分化为间充质谱系的细胞并具有广泛的增殖能力。当在二维培养物中与肝细胞共培养时，它们发挥营养作用，显著改善肝细胞存活和功能。在这项研究中，我们的目的是（i）设计一个高通量系统（HTS），以允许测试各种不同的参数细胞封装和（ii）使用这种HTS，调查是否间充质基质细胞可以有有益的影响时，肝细胞共封装在藻酸盐微珠。使用我们的HTS平台，我们观察到MSC对肝细胞行为的一些改善，随后在藻酸盐微珠中细胞功能的低通量分析中得到证实。因此，我们的研究表明，间充质基质细胞可能是一个很好的选择，以改善肝细胞微珠的功能。此外，开发的平台可用于细胞包封的HTS研究，其中几种条件（例如，细胞数目、细胞组合、藻酸盐修饰）可以容易地同时进行比较。
Hepatocyte transplantation has emerged as an alternative to liver transplant for liver disease. Hepatocytes encapsulated in alginate microbeads have been proposed for the treatment of acute liver failure, as they are able to provide hepatic functions while the liver regenerates. Furthermore, they do not require immunosuppression, as the alginate protects the hepatocytes from the recipient's immune cells. Mesenchymal stromal cells are very attractive candidates for regenerative medicine, being able to differentiate into cells of the mesenchymal lineages and having extensive proliferative ability. When co-cultured with hepatocytes in two-dimensional cultures, they exert a trophic role, drastically improving hepatocytes survival and functions. In this study we aimed to (i) devise a high throughput system (HTS) to allow testing of a variety of different parameters for cell encapsulation and (ii) using this HTS, investigate whether mesenchymal stromal cells could have beneficial effects on the hepatocytes when co-encapsulated in alginate microbeads. Using our HTS platform, we observed some improvement of hepatocyte behavior with MSCs, subsequently confirmed in the low throughput analysis of cell function in alginate microbeads. Therefore, our study shows that mesenchymal stromal cells may be a good option to improve the function of hepatocytes microbeads. Furthermore, the platform developed may be used for HTS studies on cell encapsulation, in which several conditions (e.g., number of cells, combinations of cells, alginate modifications) could be easily compared at the same time.
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影响因子：
9.7
作者：
Fonseca, Keila B.;Bidarra, Silvia J.;Barrias, Cristina C.
通讯作者：
Barrias, Cristina C.
影响因子：
4.4
作者：
Buravkova, L. B.;Andreeva, E. R.;Zhivotovsky, B.
通讯作者：
Zhivotovsky, B.
DOI：
10.3390/microarrays4020133
发表时间：
2015-03-24
期刊：
Microarrays (Basel, Switzerland)
影响因子：
--
作者：
Andersen T;Auk-Emblem P;Dornish M
通讯作者：
Dornish M
影响因子：
13.5
作者：
Lauschke, Volker M.;Vorrink, Sabine U.;Ingelman-Sundberg, Magnus
通讯作者：
Ingelman-Sundberg, Magnus
影响因子：
4.6
作者：
Lee, Charlotte A.;Dhawan, Anil;Fitzpatrick, Emer
通讯作者：
Fitzpatrick, Emer