Blue light-induced proteomic changes in etiolated Arabidopsis seedlings.
Blue light-induced proteomic changes in etiolated Arabidopsis seedlings.
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DOI：
10.1021/pr500010z
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发表时间：
2014-05-02
影响因子：
4.4
通讯作者：
Briggs, Winslow R.
中科院分区：
文献类型：
--
作者：
Deng, Zhiping;Oses-Prieto, Juan A.;Kutschera, Ulrich;Tseng, Tong-Seung;Hao, Lingzhao;Burlingame, Alma L.;Wang, Zhi-Yong;Briggs, Winslow R.
植物通过光感受器介导的生理反应来适应环境光条件，但光感受器感知和传递光信号的机制尚未完全阐明。在这里，我们使用二维差异凝胶电泳（2D DIGE）和质谱表征的黄化拟南芥幼苗短蓝光照射诱导的早期分子事件。我们观察到的光合促生素1（phototropin 1，phot 1）的磷酸化和积累的弱叶绿体运动下蓝光1（WEB 1）的膜组分后蓝光照射。在2-DE凝胶中，phot 1的两排斑点可观察到50多个斑点，在体内phot 1的N-末端和铰链1区鉴定出8个新的磷酸化Ser/Thr位点。蓝光引起phot 1的泛素化，并且phot 1的K526被鉴定为推定的泛素化位点。我们的研究表明，phot 1的翻译后修饰比以前报道的更复杂。
Plants adapt to environmental light conditions by photoreceptor-mediated physiological responses, but the mechanism by which photoreceptors perceive and transduce the signals is still unresolved. Here, we used 2D difference gel electrophoresis (2D DIGE) and mass spectrometry to characterize early molecular events induced by short blue light exposures in etiolated Arabidopsis seedlings. We observed the phosphorylation of phototropin 1 (phot1) and accumulation of weak chloroplast movement under blue light 1 (WEB1) in the membrane fraction after blue light irradiation. Over 50 spots could be observed for the two rows of phot1 spots in the 2-DE gels, and eight novel phosphorylated Ser/Thr sites were identified in the N-terminus and Hinge 1 regions of phot1 in vivo. Blue light caused ubiquitination of phot1, and K526 of phot1 was identified as a putative ubiquitination site. Our study indicates that post-translational modification of phot1 is more complex than previously reported.
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影响因子：
2.9
作者：
Harper, SM;Christie, JM;Gardner, KH
通讯作者：
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