Autologous NK cells propagated and activated ex vivo decrease senescence markers in human PBMCs.
Autologous NK cells propagated and activated ex vivo decrease senescence markers in human PBMCs.
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DOI：
10.1016/j.bbrep.2022.101380
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发表时间：
2022-12
影响因子：
2.7
通讯作者：
Gonzalez, Rafael
中科院分区：
文献类型：
--
作者：
Chelyapov, Nickolas;Nguyen, Toai;Gonzalez, Rafael
Aging is a multifactorial process involving many steps including senescence. The immune system plays a critical role in aging where chronic inflammation and senescence has been shown to be detrimental. Natural killer (NK) cells are the predominant innate lymphocyte subset that mediate various responses to include surveillance and elimination of senescent cells. Here, we use autologous propagated and activated NK (aNK) cells from 5 patients to demonstrate that aNK cells decrease senescent cells in vitro and immunosenescence in humans based on markers p16 and β-galactosidase. In addition, inflammatory cytokine panel data suggest a role for removal of immunosenescence to reduce the aging-related inflammatory response. aNK cells exhibit an enhanced ability to eliminate tumorigenic cells and senescent cells in vitro in comparison to PBMCs. Cytotoxic effects of aNK cells on senescent fibroblasts shown in time-lapsed video illustrate cooperation among attackers. Infusion of aNK cells resulted in lower expression of senescence markers (p16 and β-galactosidase) in PBMCs. After aNK cell infusion there is a decrease of proinflammatory markers, such as IL-6 and IFN-γ. aNK cells are safe, help reduce immunosenescence, and may reduce the detrimental features of aging such as inflammation.
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DOI：
10.1084/jem.20100410
发表时间：
2011-01-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cox JH;Kljavin NM;Ramamoorthi N;Diehl L;Batten M;Ghilardi N
通讯作者：
Ghilardi N
影响因子：
64.8
作者：
Baker DJ;Childs BG;Durik M;Wijers ME;Sieben CJ;Zhong J;Saltness RA;Jeganathan KB;Verzosa GC;Pezeshki A;Khazaie K;Miller JD;van Deursen JM
通讯作者：
van Deursen JM
影响因子：
5.5
作者：
Schroth J;Thiemermann C;Henson SM
通讯作者：
Henson SM
影响因子：
7.8
作者：
Prata, Larissa G P Langhi;Ovsyannikova, Inna G;Kirkland, James L
通讯作者：
Kirkland, James L
影响因子：
4.6
作者：
Bruunsgaard, H;Skinhoj, P;Pedersen, BK
通讯作者：
Pedersen, BK