Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells.
Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells.
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DOI：
10.3892/mmr.2012.857
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发表时间：
2012-06
影响因子：
3.4
通讯作者：
Soliman KF
中科院分区：
文献类型：
--
作者：
Hardaway CM;Badisa RB;Soliman KF
抗坏血酸是广泛用于对抗自由基应激的抗氧化剂化合物之一。本研究进行检查是否抗坏血酸和过氧化氢（H2O2），单独或组合，可以影响细胞活力。鼠神经母细胞瘤细胞系N2a用于绘制抗坏血酸的剂量反应曲线。观察到生理浓度（0.1 - 0.4mM）的单独抗坏血酸不引起任何细胞死亡。然而，在药理学浓度（1 - 6 mM），抗坏血酸引起剂量依赖性细胞死亡。在24 h时，50%细胞被杀死的致死浓度（LC 50）被确定为约3.141 mM抗坏血酸。H2O2单独高达300 μ M时不会导致显著的细胞死亡。在联合处理中，当用生理浓度（0.4 mM）的抗坏血酸和400 μ M的H2O2处理细胞时，与单独使用任一化合物引起的细胞死亡率相比，观察到更高的细胞死亡率。随后的实验表明，细胞死亡部分是通过细胞中总谷胱甘肽水平的丧失介导的。这些数据表明，抗坏血酸和H2O2的组合对癌细胞存活不利。需要进一步的研究来确定这些观察结果的生理意义。
Ascorbic acid is one of the antioxidant compounds widely used against free radical stress. The present study was undertaken to examine whether ascorbic acid and hydrogen peroxide (H2O2), alone or in combination, could influence cell viability. The murine neuroblastoma cell line, N2a, was used to perform a dose response curve for ascorbic acid. It was observed that ascorbic acid alone at physiological concentrations (0.1–0.4 mM) did not cause any cell death. However, at pharmacological concentrations (1–6 mM), ascorbic acid caused dose-dependent cell death. The lethal concentration at which 50% cells were killed (LC50) was determined to be approximately 3.141 mM ascorbic acid at 24 h. H2O2 up to 300 μM alone did not cause significant cell death. In the combined treatment, when the cells were treated with ascorbic acid at physiological concentrations (0.4 mM) and H2O2 at 400 μM, higher rates of cell death were observed compared to the cell death rates caused by either compound alone. Subsequent experiments revealed that cell death was partly mediated through the loss of total glutathione levels in the cells. These data suggest that the combination of ascorbic acid and H2O2 is disadvantageous for cancer cell survival. Further studies are required to ascertain the physiological significance of these observations.
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DOI：
10.1073/pnas.171318198
发表时间：
2001-08-14
影响因子：
11.1
作者：
Levine, M;Wang, YH;Morrow, J
通讯作者：
Morrow, J
影响因子：
7.4
作者：
Kramarenko, Galina G.;Wilke, Werner W.;Schafer, Freya Q.
通讯作者：
Schafer, Freya Q.
DOI：
10.1016/0015-6264(80)90164-9
发表时间：
1980-01-01
期刊：
FOOD AND COSMETICS TOXICOLOGY
影响因子：
--
作者：
STICH, HF;WEI, L;WHITING, RF
通讯作者：
WHITING, RF
影响因子：
3.8
作者：
Badisa, RB;Ayuk-Takem, LT;Walker, EH
通讯作者：
Walker, EH
影响因子：
4
作者：
SINGHAL, RL;VIJAYVARGIYA, R
通讯作者：
VIJAYVARGIYA, R