Expression of trypsin modulating oostatic factor (TMOF) in an entomopathogenic fungus increases its virulence towards Anopheles gambiae and reduces fecundity in the target mosquito.
Expression of trypsin modulating oostatic factor (TMOF) in an entomopathogenic fungus increases its virulence towards Anopheles gambiae and reduces fecundity in the target mosquito.
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胰蛋白酶调节植入剂（TMOF）在昆虫致病性真菌中的表达增加了其对冈比亚按蚊的毒力，并降低了目标蚊子中的繁殖力。
DOI：
10.1186/1756-3305-6-22
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发表时间：
2013-01-21
影响因子：
3.2
通讯作者：
Keyhani NO
中科院分区：
文献类型：
--
作者：
Kamareddine L;Fan Y;Osta MA;Keyhani NO
成蚊和幼虫通过胰蛋白酶调节泌乳因子（TMOF）来调节肠道内的食物消化，TMOF是一种由卵巢和神经内分泌系统合成的十肽激素。TMOF目前正被开发为一种杀虫剂，然而，将肽递送到蚊子体内仍然是一个重大挑战。昆虫病原真菌为利用tof靶向蚊子提供了一种手段。采用昆虫生物测定法，评价了表达埃及伊蚊tof （Bb-Aa1）的野生型和转基因白僵菌菌株对成年甘比亚按蚊和糖食成蚊的杀伤效果。与野生型亲本菌株相比，Bb-Aa1对以糖和血为食的成年冈比亚疟蚊幼虫的毒力增强。与野生型亲本相比，使用Bb-Aa1的糖蚊和血蚊的中位致死量（LD50）下降了约20%，致死量中位数（LD50）下降了约40%。在野生型和Bb-Aa1感染中，血饲蚊子的中位致死时间（LT50）值低于糖饲蚊子。然而，与野生型亲本相比，用Bb-Aa1感染糖饲和血饲蚊子的LT50值分别降低15% ~ 25%，幼虫的LT50值降低~27%。此外，感染Bb-Aa1导致目标蚊子的繁殖力急剧下降。球孢白僵菌表达Ae。与野生型菌株相比，埃及伊蚊tof对冈比亚伊蚊的毒力增强。这些数据扩大了表达蚊子特异性分子的昆虫病原真菌的范围和用途，以提高其对疾病蚊子媒介的生物控制活性。
Adult and larval mosquitoes regulate food digestion in their gut with trypsin modulating oostatic factor (TMOF), a decapeptide hormone synthesized by the ovaries and the neuroendocrine system. TMOF is currently being developed as a mosquitocide, however, delivery of the peptide to the mosquito remains a significant challenge. Entomopathogenic fungi offer a means for targeting mosquitoes with TMOF. The efficacy of wild type and transgenic Beauveria bassiana strains expressing Aedes aegypti TMOF (Bb-Aa1) were evaluated against larvae and sugar- and blood-fed adult Anopheles gambiae mosquitoes using insect bioassays. Bb-Aa1 displayed increased virulence against larvae, and sugar and blood fed adult A. gambiae when compared to the wild type parent strain. Median lethal dose (LD50) values decreased by ~20% for larvae, and ~40% for both sugar and blood-fed mosquitoes using Bb-Aa1 relative to the wild type parent. Median lethal time (LT50) values were lower for blood-fed compared to sugar-fed mosquitoes in infections with both wild type and Bb-Aa1. However, infection using Bb-Aa1 resulted in 15% to 25% reduction in LT50 values for sugar- and blood fed mosquitoes, and ~27% for larvae, respectively, relative to the wild type parent. In addition, infection with Bb-Aa1 resulted in a dramatic reduction in fecundity of the target mosquitoes. B. bassiana expressing Ae. aegypti TMOF exhibited increased virulence against A. gambiae compared to the wild type strain. These data expand the range and utility of entomopathogenic fungi expressing mosquito-specific molecules to improve their biological control activities against mosquito vectors of disease.
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DOI：
10.1073/pnas.0606146103
发表时间：
2006-12-12
影响因子：
11.1
作者：
Borovsky, Dov;Rabindran, Shailaja;DeLoof, Arnold
通讯作者：
DeLoof, Arnold
影响因子：
5.2
作者：
Keyhani NO
通讯作者：
Keyhani NO
影响因子：
3
作者：
Mnyone, Ladslaus L.;Lyimo, Issa N.;Koenraadt, Constantianus J. M.
通讯作者：
Koenraadt, Constantianus J. M.
影响因子：
3
作者：
Howard, Annabel F. V.;N'Guessan, Raphael;Takken, Willem
通讯作者：
Takken, Willem
影响因子：
56.9
作者：
Scholte, EJ;Ng'habi, K;Knols, BGJ
通讯作者：
Knols, BGJ