Mouse models for human intestinal microbiota research: a critical evaluation.
Mouse models for human intestinal microbiota research: a critical evaluation.
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DOI：
10.1007/s00018-017-2693-8
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
de Vos WM
中科院分区：
文献类型：
--
作者：
Hugenholtz F;de Vos WM
从肠道微生物群研究的早期开始，小鼠模型就被频繁地用于研究微生物与其宿主的相互作用。然而，为了将从小鼠研究中获得的知识转化为人类情况，需要考虑小鼠和人类肠道微生物群之间的主要时空相似性和差异性。这是在这里做的特别关注肠道的比较生理学，饮食模式的影响和遗传差异。详细的系统发育和宏基因组分析表明，虽然在人类和小鼠肠道中发现了许多共同的属，但这些属的丰度差异很大，总共只有4%的细菌基因被发现具有相当大的同一性。此外，小鼠菌株种类繁多，但大多数微生物群研究都是在野生型、近交系菌株及其转基因衍生物中进行的。越来越清楚的是，这些小鼠的提供者、饲养设施和遗传背景对微生物组成有重大影响，最近的实验数据说明了这一点。这可能会影响小鼠微生物群研究的可重复性及其结论。因此，未来的研究应考虑到这些因素，以真正显示饮食、基因型或环境因素对微生物组成的影响。本文的在线版本（doi:10.1007/s00018-017-2693-8）包含补充资料，仅供授权用户使用。
Since the early days of the intestinal microbiota research, mouse models have been used frequently to study the interaction of microbes with their host. However, to translate the knowledge gained from mouse studies to a human situation, the major spatio-temporal similarities and differences between intestinal microbiota in mice and humans need to be considered. This is done here with specific attention for the comparative physiology of the intestinal tract, the effect of dietary patterns and differences in genetics. Detailed phylogenetic and metagenomic analysis showed that while many common genera are found in the human and murine intestine, these differ strongly in abundance and in total only 4% of the bacterial genes are found to share considerable identity. Moreover, a large variety of murine strains is available yet most of the microbiota research is performed in wild-type, inbred strains and their transgenic derivatives. It has become increasingly clear that the providers, rearing facilities and the genetic background of these mice have a significant impact on the microbial composition and this is illustrated with recent experimental data. This may affect the reproducibility of mouse microbiota studies and their conclusions. Hence, future studies should take these into account to truly show the effect of diet, genotype or environmental factors on the microbial composition. The online version of this article (doi:10.1007/s00018-017-2693-8) contains supplementary material, which is available to authorized users.
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影响因子：
9.8
作者：
Church DM;Goodstadt L;Hillier LW;Zody MC;Goldstein S;She X;Bult CJ;Agarwala R;Cherry JL;DiCuccio M;Hlavina W;Kapustin Y;Meric P;Maglott D;Birtle Z;Marques AC;Graves T;Zhou S;Teague B;Potamousis K;Churas C;Place M;Herschleb J;Runnheim R;Forrest D;Amos-Landgraf J;Schwartz DC;Cheng Z;Lindblad-Toh K;Eichler EE;Ponting CP;Mouse Genome Sequencing Consortium
通讯作者：
Mouse Genome Sequencing Consortium
影响因子：
15.9
作者：
HOSKINS, LC;BOULDING, ET
通讯作者：
BOULDING, ET
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
12.3
作者：
Hildebrand F;Nguyen TL;Brinkman B;Yunta RG;Cauwe B;Vandenabeele P;Liston A;Raes J
通讯作者：
Raes J