Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters.
Evaluation of viral concentration methods for SARS-CoV-2 recovery from wastewaters.
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DOI：
10.1016/j.scitotenv.2020.144105
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发表时间：
2021-02-20
期刊：
影响因子：
--
通讯作者：
Oteiza JM
中科院分区：
文献类型：
--
作者：
Barril PA;Pianciola LA;Mazzeo M;Ousset MJ;Jaureguiberry MV;Alessandrello M;Sánchez G;Oteiza JM
废水流行病学是一种有用的工具，有可能作为监测社区中SARS-CoV-2存在的补充方法，并作为新冠肺炎爆发的早期警报系统。许多研究报告了污水中SARS-CoV-2的低浓度，也揭示了污水中包膜病毒浓度的方法学验证的必要性。这项研究的目的是评估废水中病毒浓缩的不同方法，并选择和改进最大限度地回收SARS-CoV-2的方案。对基于不同原理的11种浓缩技术进行了评价：负电荷膜吸附-洗脱-聚乙二醇法(方法1-2)、聚乙二醇法(方法3-7)、聚合氯化铝(PAC)絮凝法(方法8)、超滤法(方法9)、脱脂牛奶絮凝法(方法10)和负电膜吸附-洗脱法-超滤法(方法11)。为了评估这些浓缩技术的性能，使用猫杯状病毒(FCV)作为过程控制，以避免与处理SARS-CoV-2相关的风险。聚乙二醇法和聚合氯化铝絮凝法对废水中FCV的回收率分别为62.2%和45.0%。然后，对这两种方法进行了SARS-CoV-2的特异性回收试验。两种方法都能从废水中回收SARS-CoV-2，但PAC絮凝的检测下限(4.3×10~3gC/mL)低于聚乙二醇沉淀法(4.3×10~3gC/mL)。这项工作对目前用于废水中病毒浓缩的方法以及对污水中SARS-CoV-2特异性恢复的敏感性分析进行了重要的概述。这里获得的数据突显了世界卫生组织在监测社区新冠肺炎感染方面的可行性。从污水中回收SARS-CoV-2病毒的方法：聚乙二醇沉淀法和PAC絮凝法。PAC絮凝检测结果表明，污水中SARS-CoV-2的LOD为4.3×102GC/mL。PEG沉淀显示污水中SARS-CoV-2的LOD为4.3×103GC/mL。WBE用于社区新冠肺炎感染的监测是可行的。
Wastewater-based epidemiology (WBE) is a useful tool that has the potential to act as a complementary approach to monitor the presence of SARS-CoV-2 in the community and as an early alarm system for COVID-19 outbreak. Many studies reported low concentrations of SARS-CoV-2 in sewage and also revealed the need for methodological validation for enveloped viruses concentration in wastewater. The aim of this study was to evaluate different methodologies for the concentration of viruses in wastewaters and to select and improve an option that maximizes the recovery of SARS-CoV-2. A total of 11 concentration techniques based on different principles were evaluated: adsorption-elution protocols with negatively charged membranes followed by polyethylene glycol (PEG) precipitation (Methods 1–2), PEG precipitation (Methods 3–7), aluminum polychloride (PAC) flocculation (Method 8), ultrafiltration (Method 9), skim milk flocculation (Method 10) and adsorption-elution with negatively charged membrane followed by ultrafiltration (Method 11). To evaluate the performance of these concentration techniques, feline calicivirus (FCV) was used as a process control in order to avoid the risk associated with handling SARS-CoV-2. Two protocols, one based on PEG precipitation and the other on PAC flocculation, showed high efficiency for FCV recovery from wastewater (62.2% and 45.0%, respectively). These two methods were then tested for the specific recovery of SARS-CoV-2. Both techniques could recover SARS-CoV-2 from wastewater, PAC flocculation showed a lower limit of detection (4.3 × 102 GC/mL) than PEG precipitation (4.3 × 103 GC/mL). This work provides a critical overview of current methods used for virus concentration in wastewaters and the analysis of sensitivity for the specific recovery of SARS-CoV-2 in sewage. The data obtained here highlights the viability of WBE for the surveillance of COVID-19 infections in the community. Critical overview of current methods used for virus concentration in wastewaters PEG precipitation and PAC flocculation recovered SARS-CoV-2 from sewage. PAC flocculation showed a LOD of 4.3 × 102 GC/mL of SARS-CoV-2 in sewage. PEG precipitation showed a LOD of 4.3 × 103 GC/mL of SARS-CoV-2 in sewage. WBE is viable for the surveillance of COVID-19 infections in the community.
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影响因子：
4.4
作者：
Iwai, Masae;Hasegawa, Sumiyo;Shiraki, Kimiyasu
通讯作者：
Shiraki, Kimiyasu
影响因子：
2.5
作者：
Haramoto, E.;Kitajima, M.;Ohgaki, S.
通讯作者：
Ohgaki, S.
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
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冠状病毒在水和废水中的生存
影响因子：
3.4
作者：
Gundy PM;Gerba CP;Pepper IL
通讯作者：
Pepper IL
DOI：
10.1016/j.ijfoodmicro.2009.04.002
发表时间：
2009-06-01
影响因子：
5.4
作者：
Mattison, Kirsten;Brassard, Julie;Trottier, Yvon-Louis
通讯作者：
Trottier, Yvon-Louis