Amplicon Sequencing of Variable 16S rRNA from Bacteria and ITS2 Regions from Fungi and Plants, Reveals Honeybee Susceptibility to Diseases Results from Their Forage Availability under Anthropogenic Landscapes.
Amplicon Sequencing of Variable 16S rRNA from Bacteria and ITS2 Regions from Fungi and Plants, Reveals Honeybee Susceptibility to Diseases Results from Their Forage Availability under Anthropogenic Landscapes.
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DOI：
10.3390/pathogens10030381
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发表时间：
2021-03-22
期刊：
影响因子：
--
通讯作者：
Starosta AL
中科院分区：
文献类型：
--
作者：
Ptaszyńska AA;Latoch P;Hurd PJ;Polaszek A;Michalska-Madej J;Grochowalski Ł;Strapagiel D;Gnat S;Załuski D;Gancarz M;Rusinek R;Krutmuang P;Martín Hernández R;Higes Pascual M;Starosta AL
欧洲蜜蜂和亚洲蜜蜂是重要的作物传粉者。微生物组研究可以提供与环境变化和植物可利用性有关的这些昆虫的健康和适应性的复杂信息。对细菌的16S rRNA可变区和真菌和植物的内部转录间隔区（ITS）进行扩增子测序，可以鉴定代谢组。这些方法为监测从蜜蜂肠道中分离出来的未培养微生物提供了一种工具。它们还有助于监测肠道真菌的组成，有趣的是，还有昆虫收集的花粉。在这里，我们展示了来自细菌的16S rRNA扩增子测序数据，以及来自真菌和植物的ITS2区域的扩增子测序数据，这些蜜蜂来自不同时间点的人为景观，如波兰、英国、西班牙、希腊和泰国的城市地区。我们分析了蜜蜂肠道的微生物含量以及真菌和花粉。此外，以分离的DNA为模板筛选病原菌：api小虫、ceranae、bombi、木螨（Acarapis woodi）、锥虫目（Crithidia spp，即Crithidia mellificae）、Mattesia和Apicystis spp（即Apicistis bombi）等新绿虫目。我们认为，样品之间的差异主要受细菌、植物花粉和真菌的影响。此外，以糖为基础的食物喂养的蜜蜂更容易感染真菌病原体（微蝇）和新绿果碱。在大多数样本中，微孢子虫和新绿虫同时寄生于宿主蜜蜂。负载较高的真菌和细菌群如厚壁菌门（乳酸菌）；在蜜蜂微蝇和新葛林感染的蜜蜂中观察到γ-变形菌、奈瑟菌科等未识别细菌。健康的蜜蜂有更高的植物花粉负荷和细菌群，如：Orbales， Gilliamella， Snodgrassella和enterobacteraceae。最后，在整个养蜂季节，蜜蜂转变为冬季一代（寿命较长的觅食蜜蜂）对饮食扰动最敏感，因此对病原体的攻击也最敏感。蜜蜂的进化适应可能无法使它们在现代拟人化的环境中受益。
European Apis mellifera and Asian Apis cerana honeybees are essential crop pollinators. Microbiome studies can provide complex information on health and fitness of these insects in relation to environmental changes, and plant availability. Amplicon sequencing of variable regions of the 16S rRNA from bacteria and the internally transcribed spacer (ITS) regions from fungi and plants allow identification of the metabiome. These methods provide a tool for monitoring otherwise uncultured microbes isolated from the gut of the honeybees. They also help monitor the composition of the gut fungi and, intriguingly, pollen collected by the insect. Here, we present data from amplicon sequencing of the 16S rRNA from bacteria and ITS2 regions from fungi and plants derived from honeybees collected at various time points from anthropogenic landscapes such as urban areas in Poland, UK, Spain, Greece, and Thailand. We have analysed microbial content of honeybee intestine as well as fungi and pollens. Furthermore, isolated DNA was used as the template for screening pathogens: Nosema apis, N. ceranae, N. bombi, tracheal mite (Acarapis woodi), any organism in the parasitic order Trypanosomatida, including Crithidia spp. (i.e., Crithidia mellificae), neogregarines including Mattesia and Apicystis spp. (i.e., Apicistis bombi). We conclude that differences between samples were mainly influenced by the bacteria, plant pollen and fungi, respectively. Moreover, honeybees feeding on a sugar based diet were more prone to fungal pathogens (Nosema ceranae) and neogregarines. In most samples Nosema sp. and neogregarines parasitized the host bee at the same time. A higher load of fungi, and bacteria groups such as Firmicutes (Lactobacillus); γ-proteobacteria, Neisseriaceae, and other unidentified bacteria was observed for Nosema ceranae and neogregarine infected honeybees. Healthy honeybees had a higher load of plant pollen, and bacteria groups such as: Orbales, Gilliamella, Snodgrassella, and Enterobacteriaceae. Finally, the period when honeybees switch to the winter generation (longer-lived forager honeybees) is the most sensitive to diet perturbations, and hence pathogen attack, for the whole beekeeping season. It is possible that evolutionary adaptation of bees fails to benefit them in the modern anthropomorphised environment.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
64.8
作者：
Costanza, R;dArge, R;vandenBelt, M
通讯作者：
vandenBelt, M
影响因子：
3.4
作者：
GILLIAM, M;PREST, DB
通讯作者：
PREST, DB
影响因子：
1.9
作者：
Cilia, Giovanni;Fratini, Filippo;Nanetti, Antonio
通讯作者：
Nanetti, Antonio
影响因子：
4.1
作者：
Gous, Annemarie;Swanevelder, Dirk Z. H.;Willows-Munro, Sandi
通讯作者：
Willows-Munro, Sandi