Dendritic phosphorescent probes for oxygen imaging in biological systems.
Dendritic phosphorescent probes for oxygen imaging in biological systems.
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DOI：
10.1021/am9001698
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发表时间：
2009-06
影响因子：
9.5
通讯作者：
Vinogradov SA
中科院分区：
文献类型：
--
作者：
Lebedev AY;Cheprakov AV;Sakadzić S;Boas DA;Wilson DF;Vinogradov SA
生物系统中的氧水平可以通过磷光猝灭法使用具有可控猝灭参数和限定的生物分布的探针来测量。我们描述了一种通用的方法来构建磷光纳米传感器与可调光谱特性，不同程度的淬火，和高选择性的氧。该探针是基于明亮的磷光铂和钯配合物的卟啉和对称π-扩展的卟啉（四苯并卟啉和四萘卟啉）。核心大环的π-延伸允许调节探针的光谱参数以满足特定成像应用的要求（例如，氧断层摄影术与平面显微成像）。金属卟啉被封装到聚（芳基甘氨酸）树枝状聚合物，折叠在水性环境中，并创建氧气的扩散屏障，使其能够调节的灵敏度和动态范围的方法。树枝状聚合物的外围用聚（乙二醇）残基修饰，这增强了探针的溶解度，降低了毒性，并有助于防止探针与生物环境的相互作用。在生理条件下测量探针的参数，并显示不受生物大分子存在的影响。在应用中证明了探针的性能，包括大鼠脑中血管pO 2的体内显微镜检查。
Oxygen levels in biological systems can be measured by the phosphorescence quenching method using probes with controllable quenching parameters and defined biodistributions. We describe a general approach to the construction of phosphorescent nanosensors with tunable spectral characteristics, variable degrees of quenching, and a high selectivity for oxygen. The probes are based on bright phosphorescent Pt and Pd complexes of porphyrins and symmetrically π-extended porphyrins (tetrabenzoporphyrins and tetranaphthoporphyrins). π-Extension of the core macrocycle allows tuning of the spectral parameters of the probes in order to meet the requirements of a particular imaging application (e.g., oxygen tomography versus planar microscopic imaging). Metalloporphyrins are encapsulated into poly(arylglycine) dendrimers, which fold in aqueous environments and create diffusion barriers for oxygen, making it possible to regulate the sensitivity and the dynamic range of the method. The periphery of the dendrimers is modified with poly(ethylene glycol) residues, which enhance the probe’s solubility, diminish toxicity, and help prevent interactions of the probes with the biological environment. The probe’s parameters were measured under physiological conditions and shown to be unaffected by the presence of biomacromolecules. The performance of the probes was demonstrated in applications, including in vivo microscopy of vascular pO2 in the rat brain.
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影响因子：
4.2
作者：
Borisov, Sergey M.;Klimant, Ingo
通讯作者：
Klimant, Ingo
影响因子：
7.4
作者：
Borisov, S. M.;Nuss, G.;Klimant, I.
通讯作者：
Klimant, I.
影响因子：
6.1
作者：
Fernandez-Sanchez, J. F.;Roth, T.;Spichiger-Keller, U. E.
通讯作者：
Spichiger-Keller, U. E.
影响因子：
2.9
作者：
Dunphy, I;Vinogradov, SA;Wilson, DF
通讯作者：
Wilson, DF
影响因子：
4.9
作者：
Finikova, O;Cheprakov, A;Vinogradov, S
通讯作者：
Vinogradov, S