Synergistic effect of trichostatin A and scriptaid on the development of cloned rabbit embryos.
Synergistic effect of trichostatin A and scriptaid on the development of cloned rabbit embryos.
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DOI：
10.1016/j.theriogenology.2013.03.003
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发表时间：
2013-06
期刊：
影响因子：
2.8
通讯作者：
Sung LY
中科院分区：
文献类型：
--
作者：
Chen CH;Du F;Xu J;Chang WF;Liu CC;Su HY;Lin TA;Ju JC;Cheng WT;Wu SC;Chen YE;Sung LY
第一只成功的兔子SCNT是在十多年前实现的，但兔子仍然是最难克隆的物种之一。本研究旨在评价两种组蛋白去乙酰化酶抑制剂（HDACi），即阿司他丁A（TSA）和司替加（SCP），对兔克隆效率的影响。体外发育，乙酰化水平的组蛋白H4赖氨酸5（H4 K5 ac），和Oct-4蛋白表达模式的克隆胚胎进行了系统的检查后，各种HDACi处理。在培养基中补充TSA（50 nM）或SCP（250 nM）6 h与不含HDACi的处理相比提高了克隆胚胎的囊胚发育率。TSA（50 nM）和SCP（250 nM）的联合处理进一步提高了桑椹胚（58.6%）和囊胚（49.4%）率。更重要的是，与单独HDACi处理相比，联合处理的胚胎具有更高的H4 K5 ac水平和增加的总细胞数（203.7±14.4 vs 158.9±9.0或162.1±8.2，P<0.05），并且在孵化囊胚中具有更好的Oct-4表达模式，表明胚胎质量显著提高。这显然是关于Oct-4在克隆兔胚胎中表达的第一份报告。我们推测，大多数克隆兔胚胎的ICM结构异常，伴随着异常的Oct-4信号的空间分布。本研究表明TSA和SCP处理对克隆兔胚胎具有协同效应，这可能有助于提高兔克隆效率。
The first successful rabbit SCNT was achieved more than one decade ago, yet rabbits remain one of the most difficult species to clone. The present study was designed to evaluate the effects of two histone deacetylase inhibitors (HDACi), namely trichostatin A (TSA) and scriptaid (SCP), on cloning efficiency in rabbits. The in vitro development, acetylation levels of histone H4 lysine 5 (H4K5ac), and Oct-4 protein expression patterns of cloned embryos were systemically examined after various HDACi treatments. Supplementation of TSA (50 nM) or SCP (250 nM) in the culture medium for 6 h improved blastocyst development rates of cloned embryos compared to treatment without HDACi. The combined treatment with TSA (50 nM) and SCP (250 nM) further enhanced morula (58.6%) and blastocyst (49.4%) rates in vitro. More importantly, compared to single HDACi treatments, embryos with the combined treatment had a higher level of H4K5ac and an increased total cell number (203.7±14.4 vs 158.9±9.0 or 162.1±8.2, P<0.05) with better Oct-4 expression pattern in hatching blastocysts, indicating substantially improved embryo quality. This was apparently the first report regarding Oct-4 expression in cloned rabbit embryos. We inferred that the majority of cloned rabbit embryos had an aberrant ICM structure accompanied with abnormal spatial distribution of Oct-4 signals. This study demonstrated a synergistic effect of TSA and SCP treatments on cloned rabbit embryos, which may be useful to improve cloning efficiency in rabbits.
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影响因子：
4
作者：
Chen CH;Chang WF;Liu CC;Su HY;Shyue SK;Cheng WT;Chen YE;Wu SC;Du F;Sung LY;Xu J
通讯作者：
Xu J
影响因子：
10.5
作者：
Boiani, M;Eckardt, S;McLaughlin, KJ
通讯作者：
McLaughlin, KJ
DOI：
10.1089/clo.2008.0072
发表时间：
2009-03-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Meng, Qinggang;Polgar, Zsuzsanna;Dinnyes, Andras
通讯作者：
Dinnyes, Andras
影响因子：
30.8
作者：
Chan MM;Smith ZD;Egli D;Regev A;Meissner A
通讯作者：
Meissner A
影响因子：
3.6
作者：
Daniels, R;Hall, V;Trounson, AO
通讯作者：
Trounson, AO