Performing multi-step chemical reactions in microliter-sized droplets by leveraging a simple passive transport mechanism.
Performing multi-step chemical reactions in microliter-sized droplets by leveraging a simple passive transport mechanism.
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DOI：
10.1039/c7lc01009e
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发表时间：
2017-12-05
期刊：
影响因子：
6.1
通讯作者：
van Dam RM
中科院分区：
文献类型：
--
作者：
Wang J;Chao PH;Hanet S;van Dam RM
尽管正电子发射断层扫描（PET）成像在疾病的研究和临床管理中的重要性日益增加，但由于其半衰期短（需要每天生产）以及示踪剂生产的高成本和复杂性，获得无数新的放射性示踪剂受到严重限制。将基于电介质上电润湿（EWOD）的液滴微流体应用于放射化学领域可以显著减少安全所需的辐射屏蔽的量以及合成所需的前体和其他试剂的量。此外，已经观察到示踪剂的摩尔活性的显著改善。然而，该技术的广泛使用目前部分地受到原型芯片的高成本和操作复杂性的阻碍。为了解决这些问题，我们开发了一种新的微流体装置的基础上，图案化的润湿性的多步放射化学反应的微升液滴和实施自动化系统的试剂加载和收集的粗产物合成后。在本文中，我们描述了一种简单而廉价的方法来制造芯片，并证明了原型芯片进行多步放射化学反应，以产生PET示踪剂[18 F]fallypride和[18 F]FDG的可行性，并进一步表明，合成的[18 F]fallypride可用于在体小鼠成像。
Despite the increasing importance of positron emission tomography (PET) imaging in research and clinical management of disease, access to myriad new radioactive tracers is severely limited due to their short half-lives (which requires daily production) and the high cost and complexity of tracer production. The application of droplet microfluidics based on electrowetting-on-dielectric (EWOD) to the field of radiochemistry can significantly reduce the amount of radiation shielding necessary for safety, and the amount of precursor and other reagents needed for the synthesis. Furthermore, significant improvements in the molar activity of the tracers have been observed. However, widespread use of this technology is currently hinder in part by the high cost of prototype chips and operating complexity. To address these issues, we developed a novel microfluidic device based on patterned wettability for multi-step radiochemical reactions in microliter droplets and implemented automated systems for reagent loading and collection of the crude product after synthesis. In this paper, we describe a simple and inexpensive method for fabricating the chips, and demonstrate the feasibility of prototype chips for performing multi-step radiochemical reactions to produce the PET tracers [18F]fallypride and [18F]FDG, and further show that synthesized [18F]fallypride can be used for in vivo mouse imaging.
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影响因子：
4.6
作者：
Liu C;Sun J;Li J;Xiang C;Che L;Wang Z;Zhou X
通讯作者：
Zhou X
DOI：
10.1073/pnas.1117566109
发表时间：
2012-01-17
影响因子：
11.1
作者：
Keng, Pei Yuin;Chen, Supin;van Dama, R. Michael
通讯作者：
van Dama, R. Michael
影响因子：
8.4
作者：
Hong, Jiwoo;Park, Jun Kwon;Suh, Yong Kweon
通讯作者：
Suh, Yong Kweon
影响因子：
2.1
作者：
CHO, CC;WALLACE, RM;FILESSESLER, LA
通讯作者：
FILESSESLER, LA
微流体：一种用于宠物示踪剂生产的开创性技术？
DOI：
10.3390/molecules18077930
发表时间：
2013-07-05
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Rensch C;Jackson A;Lindner S;Salvamoser R;Samper V;Riese S;Bartenstein P;Wängler C;Wängler B
通讯作者：
Wängler B