Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae).
Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae).
复制标题
木本植物梭梭（藜科）和草本根全寄生虫肉苁蓉（列当科）之间的大规模 mRNA 转移
DOI：
10.1016/j.isci.2022.105880
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发表时间：
2023-01-20
期刊：
影响因子：
5.8
通讯作者：
Wuriyanghan, Hada
中科院分区：
文献类型：
--
作者：
Fan, Yanyan;Zhao, Qiqi;Lu, Huimin;Bi, Shuxin;Hao, Xiaomin;Xu, Rui;Bai, Runyao;Yu, Ruonan;Lu, Wenting;Bao, Tiejun;Wuriyanghan, Hada
寄主和根部寄生虫之间有mRNA的交换，而根部寄生虫则没有。肉桂是一种寄生于藜科植物梭梭根部的寄生草本植物。我们使用转录组测序和生物信息学分析来识别近1万个可移动的mRNA。转录本的丰度似乎是转移事件的驱动力，并且通过吸器连接进行mRNA交换。选择的mRNAs在原位和在向日葵-欧罗班幼虫异源寄生系统中得到了证实。四个肉桂→H.ammodendron移动的mRNA似乎促进吸器的发育。有趣的是，可能的抗性基因cdNLR1和cdNLR2的两个可移动的mRNAs引起根特异的过敏性反应并延缓寄生植物的发育，这可能有助于寄生平衡。本研究为木本寄主和根部寄生虫之间的大规模mRNA转移事件提供了证据，并证明了6个肉芽孢子虫基因在宿主-寄生虫相互作用中的功能相关性。以向日葵-月见草属植物系统为材料，研究了梭梭与寄生肉桂的10000个mRNAs之间的转移，并对其进行了RNA迁移和功能分析。结果表明，cdNLR1和cdNLR2会引起根特异的HR，影响寄生平衡；植物与生物体的相互作用；动态组；转录组学。
Exchanges of mRNA were shown between host and stem parasites but not root parasites. Cistanche deserticola (Orobanchaceae) is a holoparasitic herb which parasitizes on the roots of woody plant Haloxylon ammodendron (Chenopodiaceae). We used transcriptome sequencing and bioinformatic analyses to identify nearly ten thousand mobile mRNAs. Transcript abundance appears to be a driving force for transfer event and mRNA exchanges occur through haustorial junction. Mobility of selected mRNAs was confirmed in situ and in sunflower-Orobanche cumana heterologous parasitic system. Four C. deserticola →H. ammodendron mobile mRNAs appear to facilitate haustorium development. Of interest, two mobile mRNAs of putative resistance genes CdNLR1 and CdNLR2 cause root-specific hypersensitive response and retard parasite development, which might contribute to parasitic equilibrium. The present study provides evidence for the large-scale mRNA transfer event between a woody host and a root parasite, and demonstrates the functional relevance of six C. deserticola genes in host-parasite interactions. Ten thousand mRNAs transfer between Haloxylon ammodendron and root parasitic Cistanche deserticola RNA mobility and functional analysis were performed in sunflower-Orobanche cumana system CdNLR1 and CdNLR2 would cause root-specific HR and affect parasitic equilibrium Plant biology; Interaction of plants with organisms; Omics; Transcriptomics.
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影响因子：
4.6
作者：
Jamar NH;Kritsiligkou P;Grant CM
通讯作者：
Grant CM
影响因子：
56.9
作者：
Kitagawa, Munenori;Wu, Peipei;Jackson, David
通讯作者：
Jackson, David
影响因子：
9.4
作者：
LeBlanc, Megan;Kim, Gunjune;Westwood, James
通讯作者：
Westwood, James
DOI：
10.1073/pnas.97.7.3718
发表时间：
2000-03-28
影响因子：
11.1
作者：
Cutler, SR;Ehrhardt, DW;Somerville, CR
通讯作者：
Somerville, CR
影响因子：
5.1
作者：
Ikeue D;Schudoma C;Zhang W;Ogata Y;Sakamoto T;Kurata T;Furuhashi T;Kragler F;Aoki K
通讯作者：
Aoki K