Assessment of drug permeability through an ex vivo porcine round window membrane model.
Assessment of drug permeability through an ex vivo porcine round window membrane model.
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DOI：
10.1016/j.isci.2023.106789
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发表时间：
2023-06-16
期刊：
影响因子：
5.8
通讯作者：
Greenbaum, Alon
中科院分区：
文献类型：
--
作者：
Moatti, Adele;Silkstone, Dylan;Martin, Taylor;Abbey, Keith;Hutson, Kendall A.;Fitzpatrick, Douglas C.;Zdanski, Carlton J.;Cheng, Alan G.;Ligler, Frances S.;Greenbaum, Alon
将药物治疗剂递送到内耳以治疗和预防听力损失是具有挑战性的。全身递送是无效的，因为只有一小部分治疗剂到达内耳。通过圆窗膜（RWM）或耳蜗造口术进行注射的侵入性手术可能会对内耳造成损伤。另一种方法是使用鼓室内注射将药物施用到中耳中，药物主要通过RWM到达内耳。然而，RWM是一种屏障，仅可渗透少量分子。为了研究和增强RWM渗透性，我们开发了一种离体猪RWM模型，其结构和厚度与人RWM相似。该模型可存活数天，并且可以在多个时间点测量药物通过。该模型提供了一种直接的方法来开发有效和非侵入性的内耳递送方法。猪圆窗膜（RWM）在结构和厚度上模仿人类RWM从新鲜组织中提取的猪RWM模型可存活长达5天通过RWM模型可在多个时间点测量药物通过和渗透性RWM模型是测试旨在增强RWM渗透性的技术的有价值的工具给药系统;卫生技术;膜
Delivery of pharmaceutical therapeutics to the inner ear to treat and prevent hearing loss is challenging. Systemic delivery is not effective as only a small fraction of the therapeutic agent reaches the inner ear. Invasive surgeries to inject through the round window membrane (RWM) or cochleostomy may cause damage to the inner ear. An alternative approach is to administer drugs into the middle ear using an intratympanic injection, with the drugs primarily passing through the RWM to the inner ear. However, the RWM is a barrier, only permeable to a small number of molecules. To study and enhance the RWM permeability, we developed an ex vivo porcine RWM model, similar in structure and thickness to the human RWM. The model is viable for days, and drug passage can be measured at multiple time points. This model provides a straightforward approach to developing effective and non-invasive delivery methods to the inner ear. The porcine round window membrane (RWM) mimics the human RWM in structure and thickness The porcine RWM model, extracted from fresh tissue, remains viable for up to five days Drug passage and permeability can be measured at multiple time points via the RWM model The RWM model is a valued tool for testing techniques aimed at enhancing RWM permeability Drug delivery system; Health technology; Membranes
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影响因子：
15.9
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