Acidic mix of FSH isoforms are better facilitators of ovarian follicular maturation and E2 production than the less acidic.
Acidic mix of FSH isoforms are better facilitators of ovarian follicular maturation and E2 production than the less acidic.
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FSH 亚型的酸性混合物比酸性较低的混合物更能促进卵泡成熟和 E2 的产生。
DOI：
10.1210/endo.143.1.8601
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发表时间：
2002
期刊：
影响因子：
4.8
通讯作者：
V. Padmanabhan
中科院分区：
文献类型：
--
作者：
C. West;N. Carlson;James S. Lee;A. McNeilly;T. P. Sharma;W. Ye;V. Padmanabhan
FSH作为具有不同生物学属性的同种型的混合物分泌。为了确定FSH异质性的功能意义，将酸性（绵羊垂体FSH; C-FSH）和酸性较低的混合物（暴露于神经氨酸酶的C-FSH; N-FSH）给予青春期前羔羊。GnRH诱导的酸性较低的FSH的产生被竞争性GnRH受体拮抗剂Nal-Glu阻断。从Nal-Glu后24 h开始，注射C-FSH或N-FSH和LH。对照组包括未处理组、GnRH处理组和Nal-Glu处理组。每隔2小时采集血样。治疗前血浆FSH水平相似，C-FSH组随时间推移而升高，而N-FSH组则无差异（P < 0.001）。6只GnRH处理的母羊中有3只表现出LH激增。GnRH治疗动物中的E2峰浓度在治疗开始后30-36 h达到。C-FSH组的循环E2峰值水平往往高于GnRH治疗组。六只接受N-FSH治疗的母羊中只有两只血清E2升高。C-FSH组母羊的雌激素样卵泡数显著多于GnRH组和N-FSH组（P < 0.05）。我们的研究结果表明，C-FSH比N-FSH清除更慢，C-FSH比N-FSH更能促进卵泡发育和成熟。
FSH is secreted as a mix of isoforms with varying biologic attributes. To determine the functional significance of FSH heterogeneity, an acidic (ovine pituitary FSH; C-FSH) and less acidic mix (C-FSH exposed to neuraminidase; N-FSH) were administered to prepubertal lambs. Production of GnRH- induced less acidic FSH was blocked with a competitive GnRH receptor antagonist, Nal-Glu. Beginning 24 h after Nal-Glu, lambs were injected with C-FSH or N-FSH and LH. Controls included untreated, GnRH-treated, and Nal-Glu-treated groups. Blood samples were obtained at 2-h intervals. Plasma FSH levels were similar before treatment and increased over time in the C-FSH but not the N-FSH group (P < 0.001). Three of the six GnRH-treated ewes exhibited an LH surge. Peak E2 concentrations in the GnRH-treated animals were achieved 30-36 h after initiation of treatment. Peak circulating E2 levels tended to be higher in the C-FSH than in the GnRH-treated group. Only two of six N-FSH-treated ewes had a serum E2 rise. The C-FSH ewes had more estrogenic follicles than the GnRH and N-FSH groups (P < 0.05). Our findings show that C-FSH clears more slowly than N-FSH, and C-FSH is a better facilitator of follicular development and maturation than N-FSH.
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整个人类月经周期中基础和促性腺激素释放激素释放血清卵泡刺激激素电荷亚型分布的动态。
DOI：
10.1210/jcem.80.5.7745013
发表时间：
1995
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
--
作者：
Zambrano,E;Olivares,A;Mendez,JP;Guerrero,L;Diaz-Cueto,L;Veldhuis,JD;Ulloa-Aguirre,A
通讯作者：
Ulloa-Aguirre,A
影响因子：
20.3
作者：
J. Richards
通讯作者：
J. Richards
影响因子：
20.3
作者：
Alfredo Ulloa-Aguirre;A. Midgley;I. Beitins;I. Beitins;Vasantha Padmanabhan
通讯作者：
Alfredo Ulloa-Aguirre;A. Midgley;I. Beitins;I. Beitins;Vasantha Padmanabhan
评估内分泌变化的新方法：分析 GnRH 拮抗剂 (Nal-Glu) 对卵巢切除母羊促性腺激素释放的抑制。
影响因子：
5.8
作者：
Normolle,DP;Evans,NP;Dahl,GE;Padmanabhan,V
通讯作者：
Padmanabhan,V
在雌性羔羊青春期的实验诱导过程中，循环生物活性卵泡刺激激素和酸性较低的卵泡刺激激素亚型增加。
影响因子：
4.8
作者：
Padmanabhan,V;Mieher,CD;Borondy,M;I'Anson,H;Wood,RI;Landefeld,TD;Foster,DL;Beitins,IZ
通讯作者：
Beitins,IZ