Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice.
Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice.
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本研究的目的是阐明来自人类乳牙的干细胞和碳酸盐磷灰石的组合在颅骨缺损的骨再生中的效率。建立免疫缺陷小鼠(每组5只，每组5只)，建立直径5 mm的人工颅骨骨缺损模型，将人乳牙(SHODS)和碳羟基磷灰石(CAP)颗粒中的干细胞作为支架材料进行移植。移植后12周行组织学和免疫组织化学检测骨形态发生蛋白-2(BMP-2)、血管内皮生长因子(VEGF)和分化簇(CD)31的表达。在3D分析中，在SHADS和CAP中观察到了再生骨的形成，其中SHADS和CAP的组合显示出显著高于其他组的骨再生。组织学和免疫组织化学检测显示，SHEDS和CAP联合应用可增强BMP-2、VEGF和CD31的表达，促进骨再生。本研究表明，在牙槽骨缺损中，人工骨与CAP联合移植可能是一种很有前途的骨再生工具。
The objective of this study was to clarify the efficiency of a combination of stem cells from human deciduous teeth and carbonate apatite in bone regeneration of calvarial defects. Immunodeficient mice (n = 5 for each group/4 groups) with artificial calvarial bone defects (5 mm in diameter) were developed, and stem cells from human deciduous teeth (SHEDs) and carbonate hydroxyapatite (CAP) granules were transplanted with an atelocollagen sponge as a scaffold. A 3D analysis using microcomputed tomography, and 12 weeks after transplantation, histological and immunohistochemical evaluations of markers of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), and cluster of differentiation (CD) 31 were performed. In the 3D analysis, regenerated bone formation was observed in SHEDs and CAP, with the combination of SHEDs and CAP showing significantly greater bone regeneration than that in the other groups. Histological and immunohistochemical evaluations showed that combining SHEDs and CAP enhanced the expression of BMP-2, VEGF, and CD31, and promoted bone regeneration. This study demonstrates that the combination of SHEDs and CAP transplantation may be a promising tool for bone regeneration in alveolar defects.
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