Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery.
Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery.
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DOI：
10.1111/febs.14068
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Peterman EJG
中科院分区：
文献类型：
--
作者：
Prevo B;Scholey JM;Peterman EJG
鞭毛内运输（IFT）是一种依赖运动的货物运输形式，对纤毛的组装、维持和长度控制至关重要，纤毛在几乎所有真核细胞的运动、感觉接收和信号转导中起着关键作用。在IFT过程中，顺行动力蛋白-2和逆行动力蛋白马达驱动IFT列车的双向运输，将货物（例如轴素前体，如小管）以及信号转导机制的分子运送到纤毛内的组装位置。在衣藻中发现IFT后，IFT已成为研究依赖运动的货物运输一般原理的强大模型系统，我们现在认识到不同细胞类型纤毛中存在的IFT机制的多样性。例如，异三聚体激酶-2功能的缺失会导致衣单胞菌中IFT和纤毛的完全缺失，但在秀丽隐杆线虫中，其主要功能是将IFT机制加载到纤毛中，在此之后，同源二聚体激酶-2驱动的IFT会持续存在并组装全长纤毛。一般来说，异三聚体运动蛋白-2和ift -动力蛋白马达被认为作为核心ift马达发挥着广泛的作用，而同二聚体运动蛋白-2马达是在广泛的纤毛中介导不同功能的辅助马达，在某些情况下有助于轴素组装或信号分子的传递，但在许多其他情况下，它们的纤毛功能（如果有的话）仍然未知。在这篇综述中，我们着重于运动的作用机制，运动的合作和运动依赖的货物运输在IFT。鞭毛内运输是一种依赖运动的货物运输形式，对纤毛的组装、维持和长度控制至关重要，纤毛在几乎所有真核细胞的运动、感觉接收和信号转导中起着关键作用。本文综述了鞭毛内运输的运动作用、运动合作和依赖运动的货物运输机制。
Intraflagellar transport (IFT) is a form of motor-dependent cargo transport that is essential for the assembly, maintenance and length-control of cilia, which play critical roles in motility, sensory reception and signal transduction in virtually all eukaryotic cells. During IFT, anterograde kinesin-2 and retrograde IFT-dynein motors drive the bidirectional transport of IFT trains that deliver cargo, for example axoneme precursors such as tubulins as well as molecules of the signal transduction machinery, to their site of assembly within the cilium. Following its discovery in Chlamydomonas, IFT has emerged as a powerful model system for studying general principles of motor-dependent cargo transport and we now appreciate the diversity that exists in the mechanism of IFT within cilia of different cell-types. The absence of heterotrimeric kinesin-2 function, for example, causes a complete loss of both IFT and cilia in Chlamydomonas but following its loss in C. elegans, where its primary function is loading the IFT machinery into cilia, homodimeric kinesin-2-driven IFT persists and assembles a full-length cilium. Generally, heterotrimeric kinesin-2 and IFT-dynein motors are thought to play widespread roles as core IFT-motors whereas homodimeric kinesin-2 motors are accessory motors that mediate different functions in a broad range of cilia, in some cases contributing to axoneme assembly or the delivery of signaling molecules but in many other cases their ciliary functions, if any, remain unknown. In this review, we focus on mechanisms of motor action, motor cooperation and motor-dependent cargo delivery during IFT. Intraflagellar transport is a form of motor-dependent cargo transport that is essential for the assembly, maintenance and length-control of cilia, which play critical roles in motility, sensory reception and signal transduction in virtually all eukaryotic cells. In this review of intraflagellar transport, we focus on mechanisms of motor action, motor cooperation and motor-dependent cargo delivery.
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影响因子：
10.1
作者：
Brown JM;Witman GB
通讯作者：
Witman GB
影响因子：
4
作者：
Asante D;Stevenson NL;Stephens DJ
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Stephens DJ
影响因子：
19
作者：
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通讯作者：
Rosenbaum, Joel L.
DOI：
10.1099/00207713-52-2-297
发表时间：
2002-03-01
影响因子：
2.8
作者：
Cavalier-Smith, T
通讯作者：
Cavalier-Smith, T
影响因子：
9.2
作者：
Andreasson, Johan O. L.;Shastry, Shankar;Hancock, William O.;Block, Steven M.
通讯作者：
Block, Steven M.