Synthesis and Characterization of Silver-Coated Polymeric Scaffolds for Bone Tissue Engineering: Antibacterial and In Vitro Evaluation of Cytotoxicity and Biocompatibility.
Synthesis and Characterization of Silver-Coated Polymeric Scaffolds for Bone Tissue Engineering: Antibacterial and In Vitro Evaluation of Cytotoxicity and Biocompatibility.
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DOI：
10.1021/acsomega.0c05596
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发表时间：
2021-02-16
期刊：
影响因子：
4.1
通讯作者：
Amin R
中科院分区：
文献类型：
--
作者：
Khan MUA;Abd Razak SI;Mehboob H;Abdul Kadir MR;Anand TJS;Inam F;Shah SA;Abdel-Haliem MEF;Amin R
在骨组织工程中，多功能复合材料具有很强的挑战性。骨组织工程是一项创新技术，旨在开发具有良好抗菌性能和力学性能的生物相容性支架，适合骨科应用。本研究采用自由基聚合法，将阿拉伯木聚糖-共聚丙烯酸、纳米羟基磷灰石(NHAp)、纳米氧化铝(NAl_2O_3)、氧化石墨烯(GO)复合而成聚合物纳米复合支架，用于冷冻干燥法制备多孔支架。这些聚合物纳米复合支架表面包覆了银纳米颗粒，以提高抗菌活性。NHAp、nAl_2O_3和GO共同增强了材料的多功能性能，从而调节了材料的物理化学和生物力学性能。结果表明，银包覆聚合物纳米复合支架具有优良的抗菌性能和较好的微观结构性能。随着GO加入量的增加，多孔支架的形态特征和最大抑菌圈均呈现出规律性。随着GO含量的增加，纳米体系和聚合物基质提高了支架的抗压强度(18.89 Mpa)和杨氏弹性模量(198.61 Mpa)。研究了该支架对小鼠成骨前细胞系(MC3T3-E1)的生物活性，增加GO的量有助于细胞的黏附和增殖。因此，我们的研究结果表明，这些银涂层聚合物纳米复合材料支架具有工程化骨组织的潜力。
In bone tissue engineering, multifunctional composite materials are very challenging. Bone tissue engineering is an innovative technique to develop biocompatible scaffolds with suitable orthopedic applications with enhanced antibacterial and mechanical properties. This research introduces a polymeric nanocomposite scaffold based on arabinoxylan-co-acrylic acid, nano-hydroxyapatite (nHAp), nano-aluminum oxide (nAl2O3), and graphene oxide (GO) by free-radical polymerization for the development of porous scaffolds using the freeze-drying technique. These polymeric nanocomposite scaffolds were coated with silver (Ag) nanoparticles to improve antibacterial activities. Together, nHAp, nAl2O3, and GO enhance the multifunctional properties of materials, which regulate their physicochemical and biomechanical properties. Results revealed that the Ag-coated polymeric nanocomposite scaffolds had excellent antibacterial properties and better microstructural properties. Regulated morphological properties and maximal antibacterial inhibition zones were found in the porous scaffolds with the increasing amount of GO. Moreover, the nanosystem and the polymeric matrix have improved the compressive strength (18.89 MPa) and Young’s modulus (198.61 MPa) of scaffolds upon increasing the amount of GO. The biological activities of the scaffolds were investigated against the mouse preosteoblast cell lines (MC3T3-E1) and increasing the quantities of GO helps cell adherence and proliferation. Therefore, our findings showed that these silver-coated polymeric nanocomposite scaffolds have the potential for engineering bone tissue.
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DOI：
10.1016/j.ijbiomac.2017.03.067
发表时间：
2017-08-01
影响因子：
8.2
作者：
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通讯作者：
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影响因子：
3.2
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影响因子：
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影响因子：
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DOI：
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发表时间：
2018-05-01
影响因子：
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作者：
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