Measurement of the absolute immature platelet number reflects marrow production and is not impacted by platelet transfusion.
Measurement of the absolute immature platelet number reflects marrow production and is not impacted by platelet transfusion.
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DOI：
10.1111/j.1537-2995.2012.03918.x
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发表时间：
2013-06
期刊：
影响因子：
2.9
通讯作者：
Dunbar CE
中科院分区：
文献类型：
--
作者：
Bat T;Leitman SF;Calvo KR;Chauvet D;Dunbar CE
区分血小板破坏增加和血小板生成不足的能力对于骨髓衰竭综合征患者和接受高剂量化疗患者的护理非常重要。使用自动计数器根据 RNA 含量测量未成熟循环血小板现在是可行的。本研究评估了近期血小板输注对未成熟血小板参数测量的影响。使用 Sysmex XE-5000 分析仪对 9 名因再生障碍性贫血、骨髓增生异常或移植调理继发骨髓衰竭的依赖输血的患者进行血小板输注之前和之后测量未成熟血小板分数 (IPF) 和绝对未成熟血小板数量 (AIPN)。还对从正常捐献者收集的 3 种血小板去除成分进行了 5 天的储存期间的 IPF 和 AIPN 连续测量。血小板输注并没有显着改变输血患者的平均 AIPN。相比之下，IPF从-1天的6.6±4.6%显着下降到第0天的2.3±1.4%，然后在+1天返回到4.3±2.3%。在血小板成分中，AIPN 和 IPF% 在储存 5 天后显着增加，很可能是由于储存血小板的染色和检测过程存在伪影，一旦输注血小板，体内就不再检测到。由于循环血小板计数增加，血小板输注可降低 IPF。然而，血小板输注不会改变循环中的绝对未成熟血小板数量（AIPN），验证了该测定能够反映各种临床环境中骨髓持续产生的血小板，无论是否接近血小板输注。
The ability to distinguish increased platelet destruction from platelet hypo-production is important in the care of patients with bone marrow failure syndromes and patients receiving high dose chemotherapy. The measurement of immature circulating platelets based on RNA content using an automated counter is now feasible. This study evaluated the impact of recent platelet transfusion on measurement of immature platelet parameters. The immature platelet fraction (IPF) and absolute immature platelet number (AIPN) were measured using the Sysmex XE-5000 analyzer prior to and following platelet transfusion in 9 transfusion-dependent patients with marrow failure secondary to aplastic anemia, myelodysplasia or transplantation conditioning. IPF and AIPN were also measured serially over 5 days of storage in 3 plateletpheresis components collected from normal donors. Platelet transfusion did not significantly change the mean AIPN in transfused patients. In contrast, IPF decreased significantly from 6.6 ±4.6% at day -1 to 2.3 ±1.4% at day 0 before returning to 4.3 ±2.3% at day +1. In the platelet component, AIPN and IPF% increased significantly over 5 days of storage, most likely due to an artifact of the staining and detection process for stored platelets, no longer detected in vivo once the platelets were transfused. Platelet transfusion decreases the IPF due to the resultant increase in circulating platelet count. However, platelet transfusion does not change the circulating absolute immature platelet number (AIPN), validating this assay as a reflection of ongoing platelet production by the bone marrow in various clinical settings, regardless of proximity to platelet transfusion.
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影响因子：
4.8
作者：
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通讯作者：
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