Fundamentals of microfluidic cell culture in controlled microenvironments.
Fundamentals of microfluidic cell culture in controlled microenvironments.
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DOI：
10.1039/b909900j
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发表时间：
2010-03
影响因子：
46.2
通讯作者：
Beebe DJ
中科院分区：
文献类型：
--
作者：
Young EW;Beebe DJ
微流体学有可能彻底改变我们进行细胞生物学研究的方式。微流控通道的尺寸与生物细胞的物理尺度非常匹配，微流控技术的许多优点使其成为生物学新技术的一个有吸引力的平台。微流体对基础生物学的主要好处之一是能够在相应的长度和时间尺度上控制细胞微环境的参数。在用于细胞培养和后续处理和分析的新型微流控设备的设计和使用方面已经取得了相当大的进展。随着最近科学发现的步伐，评估现有工具和技术并综合将进一步提高微观生物学研究效率的基本概念变得越来越重要。本教程综述将细胞生物学和局部微环境的基本原理与细胞培养技术和微流体学的概念相结合。在微尺度环境中培养细胞需要多学科知识，包括物理、生物化学和工程学。我们讨论了与细胞的物理和生化微环境有关的基本概念、微环境的物理化学性质、细胞培养技术以及微流控设备设计和操作的实用知识。我们还讨论了微流控细胞培养的最新进展及其对该领域未来的影响。我们的目标是引导新的和感兴趣的研究人员进入科学界面临的重要领域和挑战，因为我们努力实现微流体学与生物学的完全整合。
Microfluidics has the potential to revolutionize the way we approach cell biology research. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. One of the key benefits of microfluidics for basic biology is the ability to control parameters of the cell microenvironment at relevant length and time scales. Considerable progress has been made in the design and use of novel microfluidic devices for culturing cells and for subsequent treatment and analysis. With the recent pace of scientific discovery, it is becoming increasingly important to evaluate existing tools and techniques, and to synthesize fundamental concepts that would further improve the efficiency of biological research at the microscale. This tutorial review integrates fundamental principles from cell biology and local microenvironments with cell culture techniques and concepts in microfluidics. Culturing cells in microscale environments requires knowledge of multiple disciplines including physics, biochemistry, and engineering. We discuss basic concepts related to the physical and biochemical microenvironments of the cell, physicochemical properties of that microenvironment, cell culture techniques, and practical knowledge of microfluidic device design and operation. We also discuss the most recent advances in microfluidic cell culture and their implications on the future of the field. The goal is to guide new and interested researchers to the important areas and challenges facing the scientific community as we strive toward full integration of microfluidics with biology.
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影响因子：
7.4
作者：
Heo, Yun Seok;Cabrera, Lourdes M.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
DOI：
10.1073/pnas.0610868104
发表时间：
2007-11-27
影响因子：
11.1
作者：
Huh, Dongeun;Fujioka, Hideki;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
DOI：
10.1126/science.1171643
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Discher DE;Mooney DJ;Zandstra PW
通讯作者：
Zandstra PW
影响因子：
6.1
作者：
Chen IJ;Eckstein EC;Lindner E
通讯作者：
Lindner E
DOI：
10.1073/pnas.0404353101
发表时间：
2004-11-09
影响因子：
11.1
作者：
Gu, W;Zhu, XY;Takayama, S
通讯作者：
Takayama, S