Cheek cell phospholipids in human infants: a marker of docosahexaenoic and arachidonic acids in the diet, plasma, and red blood cells.
Cheek cell phospholipids in human infants: a marker of docosahexaenoic and arachidonic acids in the diet, plasma, and red blood cells.
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人类婴儿的颊细胞磷脂：饮食、血浆和红细胞中二十二碳六烯酸和花生四烯酸的标志物。
DOI：
10.1093/ajcn/71.1.21
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
W. Connor
中科院分区：
文献类型：
--
作者：
S. Connor;N. Zhu;G. Anderson;D. Hamill;E. Jaffé;J. Carlson;W. Connor
背景
必需脂肪酸状态的评估需要采集血液或脂肪组织样本。然而，这些侵入性技术并不总是用于涉及婴儿、幼儿或难以获得血液的受试者的研究。颊粘膜（颊细胞）是一种易于接近的身体组织。
目的
目的是调查脸颊细胞的脂肪酸在多大程度上反映了血浆、红细胞和饮食中的脂肪酸含量。
设计
31名12个月大的婴儿入组。5名婴儿被喂以人乳，26名婴儿接受了提供广泛花生四烯酸和二十二碳六烯酸（DHA）摄入量的配方奶粉。通过轻轻擦拭脸颊内侧3次，将脸颊细胞收集在一小片纱布上。从纱布中提取脂质并测定颊细胞的磷脂脂肪酸含量。
结果
颊细胞磷脂中的DHA和花生四烯酸与血浆中的DHA和花生四烯酸呈显著正相关[r = 0.61（P < 0.001），r = 0.37（P <0.05）]、红细胞[r = 0.58（P < 0.001）和r = 0.37（P < 0.05）]和饮食[r = 0.65（P < 0.001）和r = 0. 51（P < 0.01）。
结论
鉴于这些相关性以及该技术的简便性和非侵入性，脸颊细胞脂肪酸可作为血浆、红细胞和饮食中必需脂肪酸含量的标志，特别是DHA和花生四烯酸。
BACKGROUND
Assessment of essential fatty acid status requires collection of blood or adipose tissue samples. However, these invasive techniques cannot always be used in studies involving infants, young children, or subjects from whom it is difficult to obtain blood. A body tissue that is easy to access is the buccal mucosa (cheek cells).
OBJECTIVE
The objective was to investigate the degree to which fatty acids of cheek cells reflect the fatty acid content of plasma, red blood cells, and the diet.
DESIGN
Thirty-one infants aged 12 mo were enrolled. Five infants were fed human milk and 26 infants received formulas that provided a wide range of arachidonic acid and docosahexaenoic acid (DHA) intakes. Cheek cells were collected on a small piece of gauze by gently swabbing the inside of the cheek 3 times. Lipids were extracted from the gauze and the phospholipid fatty acid content of the cheek cells was determined.
RESULTS
Cheek cell DHA and arachidonic acid in phospholipids were significantly correlated with DHA and arachidonic acid in plasma [r = 0.61 (P < 0.001) and r = 0.37 (P <0.05), respectively], red blood cells [r = 0.58 (P < 0.001) and r = 0.37 (P < 0.05), respectively], and the diet [r = 0.65 (P < 0.001) and r = 0. 51 (P < 0.01), respectively].
CONCLUSIONS
Given these correlations and the ease and noninvasive nature of this technique, cheek cell fatty acids may serve as a marker of the essential fatty acid content, especially of DHA and arachidonic acid, in plasma, red blood cells, and the diet.
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