Molecular and Cellular Analysis of Human T Lymphocytes Expressing γδ T‐Cell Receptor
Molecular and Cellular Analysis of Human T Lymphocytes Expressing γδ T‐Cell Receptor
复制标题
表达γT细胞受体的人T淋巴细胞的分子和细胞分析
作者：
Lorenzo Morctta;E. Ciccone;S. Ferrini;P. Pelicci;M. Mingari;J. Żeromski;C. Bottino;C. Grossi;A. Moretta
一小部分T淋巴细胞表达由γ链和δ链组成的cd3相关TCR。大多数TCR γ / δ +细胞缺乏表面CD4和CD8抗原，不与WT31单抗反应。这些阴性标准在早期研究中用于鉴定TCR γ / δ +细胞。最近，在不同的实验室中选择的针对TCR γ / δ的单克隆抗体允许直接鉴定TCR γ / δ +细胞及其亚群。发现TCR γ / δ分子在大小和电荷迁移率上是不均匀的。可以确定两种主要形式的TCR γ / δ，其特征是存在或不存在链间二硫键。生化分析最初表明，与BB3或δ TCS1/A13单抗的反应性与二硫化物（C γ 1编码）或非二硫化物连接（C γ 2编码）形式的TCR γ / δ的表达存在精确的相关性。然而，最近的研究表明，这些单抗分别与V δ 2或V δ 1的分子产物反应，针对一种或另一种形式的TCR γ / δ的单抗激活细胞的功能程序，导致细胞内Ca++的动员，淋巴因子的产生和裂解机制的触发。对TCR γ / δ介导的识别靶分子的分析表明，至少一些TCR γ / δ +细胞能够对（allo）抗原产生特异性反应，并且可以识别I类分子的多态性决定因素（如转染HLA-24等位基因的P815细胞的特异性裂解所示）。与TCR α / β +细胞不同，TCR γ / δ +细胞均由细胞溶解前体组成，这一点在凝集素依赖性和重定向杀伤试验中对大量克隆的分析中得到了证实。尽管它们的LGL形态，新鲜分离的TCR γ / δ +细胞不裂解nk敏感靶标，但暴露于il -2后会裂解。然而，在重定向杀伤实验中，抗tcr /CD3单抗也可以在新鲜细胞中诱导适度的细胞溶解活性。对表达不同TCR γ / δ类型的亚群分布的分析表明，BB3+细胞在外周血中普遍存在，在胸腺中几乎不存在；相反，A13+ (δ TCS1+)细胞占TCR γ / δ胸腺细胞的大多数。新鲜TCR γ / δ +细胞的电镜分析显示，细胞质延伸，含有大量电子致密颗粒，可识别为原代溶酶体。在IL-2的刺激下，TCR γ / δ +细胞与其他LAK细胞相似，其细胞质颗粒增加，细胞骨架结构重新分布。（摘要删节为400字）
A minor subset of T lymphocytes expresses a CD3-associated TCR composed of gamma and delta chains. The majority of TCR gamma/delta+ cells lack surface CD4 and CD8 antigen and do not react with WT31 mAb. These negative criteria were utilized in early studies to identify TCR gamma/delta+ cells. More recently, mAb to TCR gamma/delta, selected in different laboratories, have permitted the direct identification of TCR gamma/delta+ cells and their subsets. TCR gamma/delta molecules were found to be heterogeneous in size and charge mobility. Two major forms of TCR gamma/delta could be identified that are characterized by the presence or absence of an inter-chain disulphide bond. Biochemical analysis originally suggested that a precise correlation existed between reactivity with BB3 or delta TCS1/A13 mAb and expression of a disulphide (C gamma 1-encoded) or non-disulphide linked (C gamma 2-encoded) form of TCR gamma/delta. However, more recent studies have indicated that these mAb react with the molecular product of V delta 2 or V delta 1, respectively, mAb directed to one or another form of TCR gamma/delta activate the functional program of the cell, leading to intracellular Ca++ mobilization, lymphokine production and triggering of the lytic machinery. Analysis of the target molecules for TCR gamma/delta-mediated recognition revealed that at least some TCR gamma/delta+ cells are capable of specific responses to (allo)antigen and that polymorphic determinants of class I molecules can be recognized (as shown by the specific lysis of P815 cells transfected with HLA-24 allele). Unlike TCR alpha/beta+ cells, TCR gamma/delta+ cells are homogeneously composed of cytolytic precursors, as shown by the analysis of a large panel of clones in both lectin-dependent and redirected killing assays. In spite of their LGL morphology, freshly isolated TCR gamma/delta+ cells do not lyse NK-sensitive targets but do so after exposure to rIL-2. A modest cytolytic activity, however, could be induced also in fresh cells by anti-TCR/CD3 mAb in a redirected killing assay. Analysis of the distribution of the subsets expressing different TCR gamma/delta types showed that BB3+ cells are prevalent in the peripheral blood and virtually absent in the thymus; in contrast, A13+ (delta TCS1+) cells represent the majority of TCR gamma/delta+ thymocytes. Electron microscopic analysis of fresh TCR gamma/delta+ cells showed an extended cytoplasm containing numerous electron-dense granules identifiable as primary lysosomes. Upon stimulation with IL-2, TCR gamma/delta+ cells, similar to other LAK cells, display an increase in their cytoplasmic granules together with a redistribution of cytoskeletal structures.(ABSTRACT TRUNCATED AT 400 WORDS)
登录
查看更多内容
Thy-1 树突状表皮细胞属于 T 细胞谱系。
DOI：
10.1073/pnas.84.8.2430
发表时间：
1987
影响因子：
11.1
作者：
Stingl,G;Gunter,KC;Tschachler,E;Yamada,H;Lechler,RI;Yokoyama,WM;Steiner,G;Germain,RN;Shevach,EM
通讯作者：
Shevach,EM
人类 T γ δ 细胞的组织定位和 CD8 辅助分子表达。
DOI：
--
发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bucy,RP;Chen,CL;Cooper,MD
通讯作者：
Cooper,MD
由不同的 C γ 基因编码的结构不同的人类 T 细胞受体 γ 蛋白。
DOI：
10.1126/science.2955517
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Krangel,MS;Band,H;Hata,S;McLean,J;Brenner,MB
通讯作者：
Brenner,MB
影响因子：
4.4
作者：
R. P. Bucy;C.-L. H. Chen;J. Cihak;U. Lösch;M D Cooper
通讯作者：
R. P. Bucy;C.-L. H. Chen;J. Cihak;U. Lösch;M D Cooper
人类 T 细胞伽玛基因包含 N 个片段，并具有显着的连接变异性。
影响因子：
64.8
作者：
Quertermous,T;Strauss,W;Murre,C;Dialynas,DP;Strominger,JL;Seidman,JG
通讯作者：
Seidman,JG