Different levels of alternative splicing among eukaryotes.
Different levels of alternative splicing among eukaryotes.
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DOI：
10.1093/nar/gkl924
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发表时间：
2007
影响因子：
14.9
通讯作者：
Ast G
中科院分区：
文献类型：
--
作者：
Kim E;Magen A;Ast G
可变剪接增加了转录组和蛋白质组的多样性。先前旨在比较不同生物之间可变剪接速率的分析得出了相互矛盾的结果。这些矛盾的结果归因于这样一个事实，即这两种分析都依赖于表达序列标签（EST）的覆盖度，而在被检测的生物之间，这种覆盖度差异很大。在这项研究中，我们比较了八种不同生物的可变剪接水平。通过采用一种不依赖EST的方法，我们揭示出与无脊椎动物相比，脊椎动物中经历可变剪接的基因和外显子的百分比更高。我们还发现，与组成型外显子相比，跳跃型可变外显子两侧的内含子更长，而可变5′和3′剪接位点事件通常并非如此。此外，尽管在后生动物进化过程中可变剪接的调控以及内含子和外显子的大小发生了变化，但内含子保留仍然是最罕见的可变剪接类型，而外显子跳跃更为普遍，并且从无脊椎动物到脊椎动物呈现出轻微的增加。可变剪接水平的差异表明，可变剪接可能对哺乳动物更高水平的表型复杂性有很大贡献，并且内含子的积累赋予了一种进化优势，因为它允许增加可变剪接形式的数量。
Alternative splicing increases transcriptome and proteome diversification. Previous analyses aiming at comparing the rate of alternative splicing between different organisms provided contradicting results. These contradicting results were attributed to the fact that both analyses were dependent on the expressed sequence tag (EST) coverage, which varies greatly between the tested organisms. In this study we compare the level of alternative splicing among eight different organisms. By employing an EST independent approach we reveal that the percentage of genes and exons undergoing alternative splicing is higher in vertebrates compared with invertebrates. We also find that alternative exons of the skipping type are flanked by longer introns compared to constitutive ones, whereas alternative 5′ and 3′ splice sites events are generally not. In addition, although the regulation of alternative splicing and sizes of introns and exons have changed during metazoan evolution, intron retention remained the rarest type of alternative splicing, whereas exon skipping is more prevalent and exhibits a slight increase, from invertebrates to vertebrates. The difference in the level of alternative splicing suggests that alternative splicing may contribute greatly to the mammal higher level of phenotypic complexity, and that accumulation of introns confers an evolutionary advantage as it allows increasing the number of alternative splicing forms.
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影响因子：
14.9
作者：
Pruitt KD;Tatusova T;Maglott DR
通讯作者：
Maglott DR
影响因子：
10.7
作者：
Adkins, RM;Gelke, EL;Honeycutt, RL
通讯作者：
Honeycutt, RL
DOI：
10.1073/pnas.0602039103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Wang, BB;Brendel, V
通讯作者：
Brendel, V
影响因子：
7
作者：
Gerhard, DS;Wagner, L;Collins, FS
通讯作者：
Collins, FS
影响因子：
56.9
作者：
Raible, F;Tessmar-Raible, K;Arendt, D
通讯作者：
Arendt, D