A canine model of femoral head osteonecrosis induced by an ethanol injection navigated by a novel template.
A canine model of femoral head osteonecrosis induced by an ethanol injection navigated by a novel template.
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由新型模板导航的乙醇注射诱导的犬股骨头坏死模型。
DOI：
10.7150/ijms.6314
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发表时间：
2013
影响因子：
3.6
通讯作者：
Guo Z
中科院分区：
文献类型：
--
作者：
Wang C;Wang J;Zhang Y;Yuan C;Liu D;Pei Y;Li X;Wu Z;Li Y;Guo Z
如何建立大型哺乳动物股骨头坏死（ONFH）模型目前尚无共识。本研究的目的是研究一种新的犬ONFH模型的有效性，通过导航注射无水乙醇诱导。利用三维重建和快速原型制造技术，设计并加工了一种新的模板来导航乙醇注入。18只成年犬股骨头内注射无水乙醇。术后3、6、9周行大体、X线及组织学检查。此外，术后6周进行计算机断层扫描（CT）、磁共振成像（MRI）和放射性核素扫描。术后3周，股骨头显示出骨坏死的证据，包括空陷窝数量增加、造血细胞减少和髓腔内脂肪组织破坏，在随后的6周和9周随访评价中严重程度增加。术后9周观察到骨折的小梁和纤维组织。图像分析还显示了骨坏死的证据，如X线片上的几个骨质减少区域伴硬化边缘，CT扫描上的几个低骨矿物质密度区域伴硬化，MRI上的核素物质摄取增加，放射性同位素图像上的不均匀长T2信号。通过我们的新模板引导的乙醇注射成功地建立了犬ONFH模型。该模型可用于测试人类ONFH的新治疗模式。
There is no consensus on how to establish models of osteonecrosis of the femoral head (ONFH) in large mammals. The aim of this study was to investigate the effectiveness of a novel canine model of ONFH, induced by a navigated injection of absolute ethanol. Using three-dimensional reconstruction and rapid prototyping manufacturing techniques, a new template was designed and processed to navigate the ethanol injection. The femoral heads of 18 adult dogs were injected with ethanol. Macroscopic, X-ray and histological examinations were performed at 3, 6, and 9 weeks after the operation. Further, computed tomography (CT), magnetic resonance imaging (MRI), and radionuclide scans were performed 6 weeks postoperatively. Three weeks after the operation, the femoral heads showed evidence of osteonecrosis including increasing numbers of empty lacunae, decreased hematopoietic cells, and destroyed adipose tissue in the medullary cavity, which increased in severity at the subsequent follow-up evaluations at 6 and 9 weeks. Fractured trabeculae and fibrous tissue were noted 9 weeks postoperatively. Image analysis also revealed evidence of osteonecrosis, such as several osteopenic areas with sclerotic rims on the X-ray, several areas of low bone mineral density with sclerosis on the CT scan, increased uptake of the nuclide species in MRI, and an inhomogeneous long T2 signal on the radioisotopic images. Ethanol injection navigated by our novel template was successful in establishing a canine model of ONFH. This model can be used to test new treatment modalities for human ONFH.
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影响因子：
2.8
作者：
Yang, Lei;Boyd, Kelli;Kaste, Sue C.;Kamdem, Landry Kamdem;Rahija, Richard J.;Relling, Mary V.
通讯作者：
Relling, Mary V.
影响因子：
2.4
作者：
Manggold, J;Sergi, C;Simank, HG
通讯作者：
Simank, HG
影响因子：
2.4
作者：
Reed, KL;Brown, TD;Conzemius, MG
通讯作者：
Conzemius, MG
DOI：
10.1302/0301-620x.81b4.9393
发表时间：
1999-07-01
影响因子：
--
作者：
Sugano, N;Kubo, T;Ninomiya, S
通讯作者：
Ninomiya, S
影响因子：
3
作者：
Lu, Sheng;Xu, Yong Q.;Zhang, Yuan Z.
通讯作者：
Zhang, Yuan Z.